Investigation of various structures of DNA molecules (III) : Coil-globe transition of lambda-DNA induced by cationic surfactant

Science in China. Series C, Life Sciences
X FengC Bai

Abstract

The structure transition of lambda-DNA induced by cationic surfactant cellar media was investigated by using CD, SEM and AFM. The experimental data of CD revealed that lambda-DNA can be induced from B-form to a collapsed structure with the addition of the cationic surfactant CTAB to the system. The condensed process of lambda-DNA from coil state to small globular state (diameter about 1.25 microm) and finally big globular state (diameter about 5.4 microm) was observed by using SEM and AFM.

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