Investigation of water-insoluble hydrophobic polyethylenimines as RNAi vehicles in chronic myeloid leukemia therapy.

Journal of Biomedical Materials Research. Part a
Aysha S AnsariHasan Uludag

Abstract

The discovery of RNA interference (RNAi) more than two decades ago opened avenues for avant-garde cancer treatments that possess the ability to evade issues hampering current chemotherapeutic strategies, owing to its specific gene sequence-driven mechanism of action. A potent short interfering RNA (siRNA) delivery vehicle designed to overcome physiological barriers is imperative for successful RNAi therapy. For this purpose, this study explored the characteristics and therapeutic efficacy of low-molecular weight (MW) polyethylenimine (PEI) with high cholesterol substitution, yielding water-insoluble polymers, in chronic myeloid leukemia (CML) K562 cells. A strong impact of cholesterol grafting on the physicochemical attributes of the resultant polymers and their corresponding complexes with siRNA was observed, with the siRNA binding capacity of polymers increasing and complex dissociation sensitivity decreasing with increase in cholesterol content of the polymers. The modified polymer complexes were significantly smaller in size and possessed higher cationic charge compared to the parent polymer. The interaction with anionic heparan sulfate preoteoglycans present on the cell surface was significant in cellular uptake of the com...Continue Reading

References

Oct 15, 1976·International Journal of Cancer. Journal International Du Cancer·E KleinF Vánky
Jul 9, 1999·Journal of Biomedical Materials Research·W T GodbeyA G Mikos
Dec 10, 1999·Experimental Cell Research·T AokiT Fujimoto
Mar 7, 2003·Nature·Sean D Conner, Sandra L Schmid
Mar 14, 2003·Trends in Biochemical Sciences·Mattias Belting
May 3, 2003·Nature Reviews. Genetics·Clare E ThomasMark A Kay
Dec 3, 2003·Journal of Virology·Saeed Shafti-KeramatReinhard Kirnbauer
Apr 22, 2004·RNA·Juliana M LayzerBruce A Sullenger
Apr 28, 2004·Advanced Drug Delivery Reviews·S A WissingR H Müller
Jul 9, 2004·The Journal of Gene Medicine·Idit KopatzJean-Paul Behr
Sep 21, 2004·Blood·Jorge CortesHagop Kantarjian
Nov 3, 2004·Nucleic Acids Research·Raymond M SchiffelersMartin C Woodle
Mar 22, 2005·Nature Biotechnology·Adam D JudgeIan MacLachlan
Aug 5, 2005·Gene Therapy·L K Medina-KauweS Hamm-Alvarez
Nov 8, 2005·Nature Biotechnology·Joao T Marques, Bryan R G Williams
Mar 2, 2006·Pharmacological Reviews·Ikramy A KhalilHideyoshi Harashima
Jul 11, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Kenneth A HowardJørgen Kjems
Aug 15, 2006·Biomacromolecules·Nathan P Gabrielson, Daniel W Pack
Sep 12, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Anne M DoodyDavid Putnam
Sep 15, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Marjan FretzArwyn T Jones
Oct 20, 2006·Journal of Nanoscience and Nanotechnology·Mahesh D ChavanpatilJayanth Panyam
Jun 17, 2008·Bioconjugate Chemistry·Arkadi ZintchenkoErnst Wagner
Jun 30, 2009·Expert Opinion on Drug Delivery·D JereC S Cho
Dec 17, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Dries VercauterenKevin Braeckmans
Feb 27, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Myung Sook HuhIck Chan Kwon
Apr 21, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yamini ArthanariConstantinos Demonacos
Aug 17, 2010·Nanomedicine : Nanotechnology, Biology, and Medicine·Albert Kwok, Stephen L Hart
Dec 22, 2010·AAPS PharmSciTech·Surajit Das, Anumita Chaudhury
Feb 16, 2011·Macromolecular Bioscience·Hamidreza Montazeri AliabadiHasan Uludağ
Mar 22, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Md Nasimuzzaman, Derek A Persons
Jul 25, 2012·Nature Reviews. Cancer·Thomas O'HareMichael W Deininger
Jun 4, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Juliana Valencia-SernaHasan Uludağ

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