Investigation of Wolbachia spp. and Spiroplasma spp. in Phlebotomus species by molecular methods.

Scientific Reports
Bilge KaratepeMustafa Karatepe

Abstract

The aim of this study was to determine the presence of Wolbachia spp. and Spiroplasma spp. in natural populations of sand flies in Turkey by molecular methods. A total of 40 Phlebotomus specimens (19 female and 21 male) were used in this study. Genomic DNA from whole sand flies was isolated and Wolbachia spp. infection prevalence was investigated by using Wolbachia gene specific primer sets (wsp and GroEL). In addition, the DNA were analyzed for the presence of Spiroplasma infections utilizing bacterium specific 16 S rDNA PCR-amplification primers. Results of this analysis showed a Wolbachia infection prevalence of 70% (28/40). There was no sex-bias in infection prevalence, being 76% (16/21) and 63% (12/19) in males and females, respectively. Analysis of Spiroplasma infections indicated that 26% (5/19) of female sand flies were positive for infection, while none of the screened males (0/21) were positive. Of the 40 sand fly samples, only 2 were found to be positive for both Wolbachia spp. and Spiroplasma spp. The present study demonstrates the presence of Wolbachia and Spiroplasma infections in the natural sand fly populations in Turkey. This is the first report on Spiroplasma infection in the sand flies from Turkey.

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Citations

Jun 4, 2020·Pathogens·Christos PapadopoulosDimitrios G Karpouzas
Dec 21, 2021·PLoS Neglected Tropical Diseases·Rafael J Vivero-GomezClaudia Ximena Moreno-Herrera
Nov 29, 2020·Veterinary Parasitology·Mukund MadhavPeter James

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Datasets Mentioned

BETA
KC668323
as
KY523672
AE017196
and
CP003884
KT983889.1
MH393924
MH393925
MH393926

Methods Mentioned

BETA
PCR

Software Mentioned

ArcGIS
BLAST
Gel Doc EQ
Image Lab
BLASTN

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