Investigation of work of adhesion of biological cell (human hepatocellular carcinoma) by AFM nanoindentation

Journal of Micro-bio Robotics
Xinyao ZhuX Liu

Abstract

In this study, we presented an investigation of mechanical properties by AFM nanoindentation on human hepatocellular carcinoma cells treated with fullerenol for 24, 48 and 72 h. AFM nanoindentation was routinely applied to investigate the morphology and biomechanical properties of living carcinoma cells, and adhesion phenomena (negative force) were detected in the obtained force-displacement curves. Conventionally, Hertz contact model has been widely used for determination of cell elasticity, however this contact model cannot account for adhesion. Alternatively, JKR contact model, as expected for adhesion circumstance, has been applied to fit the obtained force-displacement curves. In this investigation, we have derived both the work of adhesion and the elastic modulus of biological cells (human hepatocellular carcinoma) under fullerenol treatment. The results show that the chosen JKR model can provide better fitting results than Hertz contact model. The results show that both Young's modulus and work of adhesion exhibit significant variation as the treatment time increases. The calculated mechanical properties of elastic modulus and work of adhesion can be used as an effective bio-index to evaluate the effects of fullerenol or...Continue Reading

References

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Dec 22, 2015·Journal of the Mechanical Behavior of Biomedical Materials·Xinyao ZhuX Liu

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Citations

Oct 22, 2019·The Journal of Alternative and Complementary Medicine : Research on Paradigm, Practice, and Policy·Jing ZhouMing Hu
Sep 21, 2018·Current Opinion in Otolaryngology & Head and Neck Surgery·Mosaad Abdel-AzizAmr El-Badrawy

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Methods Mentioned

BETA
AFM

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Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

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