Investigation of XoxF methanol dehydrogenases reveals new methylotrophic bacteria in pelagic marine and freshwater ecosystems

FEMS Microbiology Ecology
Arthi Ramachandran, David A Walsh

Abstract

The diversity and distribution of methylotrophic bacteria have been investigated in the oceans and lakes using the methanol dehydrogenase mxaF gene as a functional marker. However, pelagic marine (OM43) and freshwater (LD28 and PRD01a001B) methylotrophs within the Betaproteobacteria lack mxaF, instead possessing a related xoxF4-encoded methanol dehydrogenase. Here, we developed and employed xoxF4 as a complementary functional gene marker to mxaF for studying methylotrophs in aquatic environment. Using xoxF4, we detected OM43-related and LD28-related methylotrophs in the ocean and freshwaters of North America, respectively, and showed the coexistence of these two lineages in a large estuarine system (St Lawrence Estuary). Gene expression patterns of xoxF4 supported a positive relationship between xoxF4-containing methylotroph activity and spring time productivity, suggesting phytoplankton blooms are a source of methylotrophic substrates. Further investigation of methanol dehydrogenase diversity in pelagic ecosystems using comparative metagenomics provided strong support for a widespread distribution of xoxF4 (as well as several distinct xoxF5) containing methylotrophs in marine and freshwater surface waters. In total, these resu...Continue Reading

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Citations

Jul 1, 2016·World Journal of Microbiology & Biotechnology·Ludmila Chistoserdova
Jan 7, 2018·Applied and Environmental Microbiology·Jeremy D SemrauSukhwan Yoon
Jul 19, 2018·Dalton Transactions : an International Journal of Inorganic Chemistry·Henning LumpeLena J Daumann
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Sep 5, 2019·International Journal of Systematic and Evolutionary Microbiology·Wen-Ming ChenShih-Yi Sheu
Aug 20, 2019·International Journal of Systematic and Evolutionary Microbiology·Ceshing SheuWen-Ming Chen
Mar 17, 2020·International Journal of Systematic and Evolutionary Microbiology·Haoxin LvAkio Tani
Oct 10, 2020·FEMS Microbiology Letters·Jennifer B GlassKaren H Johannesson
Feb 7, 2021·FEMS Microbiology Ecology·Paula Roszczenko-JasińskaRenata Matlakowska
Feb 16, 2021·Environmental Microbiology·Peter Q FischerDiana Z Sousa
Aug 29, 2021·Science Advances·Samuel ChaffronDamien Eveillard

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