Investigation on apoptosis of neuronal cells induced by Amyloid beta-Protein

Journal of Zhejiang University. Science
Ben-Yan RuoMin Tang

Abstract

To construct a PC12 cell strain with neuronal differentiation, and observe the apoptosis and proliferation activity effects induced these cells by Amyloid beta-Protein (Abeta-43). 1) PC12 cells in logarithmic growth phase were subcultured for 24 h. After the culture fluid was changed, the cells were treated with Rat-beta-NGF and cultured for 9 days. 2) Neuronal differentiation of PC12 cells in logarithmic growth phase were divided into four groups: control group (0), experimental group (1), experimental group (2) and experimental group (3). The concentrations of Abeta in the four groups were 0 micromol/L, 1.25 micromol/L, 2.5 micromol/L and 5 micromol/L, respectively. The cells were harvested at 24, 48 and 72 h later and stained with AnnexinV-FITC/PI after centrifugation and washing. Then flow cytometry was conducted to examine the apoptosis percentage. 3) NGF-induced PC12 cells were selected and Abeta with different concentrations was added. The final concentrations of Abeta were 0 micromol/L, 1.25 micromol/L, 2.5 micromol/L and 5 micromol/L, respectively. After the cells were incubated in an atmosphere of 5% CO2 at 37 degrees C in an incubator for 72 h, the OD values were examined. 1) Neuronal differentiated PC12 cell lines w...Continue Reading

Citations

Sep 19, 2018·PloS One·Son Tung NgoHuong Thi Thu Phung
Nov 4, 2008·Neuroreport·Nikolaos PapageorgiouDesmond J Tobin

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis