Investigation on damage of BSA molecules under irradiation of low frequency ultrasound in the presence of FeIII-tartrate complexes

Ultrasonics Sonochemistry
Jun WangXiangdong Zhang

Abstract

The interaction between bovine serum albumin (BSA) and Fe(III)-tartrate complexes ([Fe(III)(tar)(H(2)O)(3)](-) and [Fe(III)(tar)(2)](5-)) as well as the damage of BSA in the presence of Fe(III)-tartrate complexes under ultrasonic irradiation was studied by UV-vis and fluorescence spectra. In addition, the influences of ultrasonic irradiation time, Fe(III)-tartrate complex concentration, ionic strength and solution acidity (pH value) were also examined on the damage of BSA. The results showed that the fluorescence quenching of BSA caused by the Fe(III)-tartrate complexes belonged to the static quenching. The BSA and Fe(III)-tartrate complexes interacted with each other mainly through weak interaction and coordinate actions. The corresponding binding association constants (K) and the binding site numbers (n) were calculated. The results were as follows: K(1)=1.67 x 10(3) L mol(-1) and n(1)=0.9699 for [Fe(III)(tar)(H(2)O)(3)](-), K(2)=1.54 x 10(3) L mol(-1) and n(2)=0.8754 for [Fe(III)(tar)(2)](5-). Otherwise, under ultrasonic irradiation the BSA molecules were obviously damaged by the Fe(III)-tartrate complexes. The damage degree rose up with the increase of ultrasonic irradiation time, Fe(III)-tartrate complex concentration, pH ...Continue Reading

References

Mar 1, 1990·Japanese Journal of Cancer Research : Gann·N YumitaS Umemura
Sep 1, 1990·Japanese Journal of Cancer Research : Gann·S UmemuraK Umemura
Mar 1, 1989·Japanese Journal of Cancer Research : Gann·N YumitaS Umemura
Jan 1, 1995·The Journal of the Acoustical Society of America·R J JeffersC A Cain
Aug 1, 1994·International Journal of Radiation Biology·D KesselC Cain
May 1, 1993·Japanese Journal of Cancer Research : Gann·S UmemuraR Nishigaki
Apr 19, 1996·Cancer Letters·K UmemuraS Umemura
Mar 1, 1996·International Journal of Radiation Biology·N YumitaR Nishigaki
Jan 1, 1996·Annual Review of Biochemistry·H B Gray, J R Winkler
Jan 1, 1996·Progress in Nucleic Acid Research and Molecular Biology·D M PerrinD S Sigman
Sep 1, 1996·The International Journal of Biochemistry & Cell Biology·A A Moosavi-MovahediP Ghadam
Jan 1, 1997·The Journal of the Acoustical Society of America·S UmemuraK Sasaki
Feb 1, 1997·Lancet·K TachibanaE Morioka
Jan 1, 1997·Methods in Enzymology·E TerpetschnigJ R Lakowicz
Jan 1, 1997·Ultrasound in Medicine & Biology·A E WorthingtonJ W Hunt
Jun 9, 1998·Japanese Journal of Cancer Research : Gann·K SasakiS Umemura
May 5, 1999·Lancet·K TachibanaK Tamura
Dec 14, 1999·Japanese Journal of Cancer Research : Gann·N SakusabeT Suzuki
Aug 30, 2001·Progress in Nucleic Acid Research and Molecular Biology·S M Cohen, S J Lippard
Dec 26, 2001·Chemical Reviews·K E ErkkilaJ K Barton
Mar 20, 2004·Ultrasonics Sonochemistry·Tinghe YuTimothy J Mason
Nov 30, 2006·Journal of Hazardous Materials·Yao-Hui HuangChuh-Yung Chen

❮ Previous
Next ❯

Citations

Oct 25, 2016·Journal of Nanoparticle Research : an Interdisciplinary Forum for Nanoscale Science and Technology·Sulena PradhanInger Odnevall Wallinder
Sep 4, 2012·Dose-response : a Publication of International Hormesis Society·Anoop K PalDonald K Milton
Mar 15, 2015·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Jyotsana PathakH B Bohidar

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.