Investigation on possible transformations of cortisol, cortisone and cortisol glucuronide in bovine faecal matter using liquid chromatography-mass spectrometry

Steroids
F ArioliG Pompa

Abstract

Given the close resemblance of the ring A structure of prednisolone and prednisone on the one hand, and of androstadienedione on the other, the transformation of cortisol and cortisone into prednisolone and prednisone in cattle faeces was evaluated. A simple method that does not involve extraction but only the 1:100 dilution of cattle faeces, spiking with 400ng/mL cortisol, cortisone or cortisol glucuronide and incubation of the suspension, was used. The analyses were performed by HPLC-MS(3) to detect the supposed Delta(1) dehydrogenation of the glucocorticoids. The decision limits (CCalpha) and detection capabilities (CCbeta) were 2.0 and 3.0ng/mL for cortisol, cortisone and prednisolone, 3.0 and 4.0ng/mL for cortisol glucuronide and 7.0 and 10.0ng/mL for prednisone, respectively. Intra-day and inter-day coefficients of variation (CV%), were 5.6-6.2 and 5.2-6.6 for cortisol glucuronide, cortisol, cortisone and prednisolone, and 16.0 and 16.2 for prednisone, respectively. The recoveries were in the range 110-143% for all analytes. Regression coefficients (R2) were in the range 0.996-0.999 for all analytes. The results show the hydrolysis of the conjugated form and the dehydrogenation in ring A in diluted faeces. It is therefore...Continue Reading

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Citations

Sep 12, 2012·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·M VincentiC Nebbia
Mar 21, 2013·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·M LeporatiM Vincenti
Apr 10, 2013·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Luigi BertocchiFrancesco Arioli
May 9, 2012·Animal : an International Journal of Animal Bioscience·F ArioliG Pompa
May 18, 2012·Water Research·Shan LiuHua-Jie Lai
Feb 14, 2013·Environmental Toxicology and Chemistry·Omer Sid-AhmedErich Möstl
Jan 15, 2014·Journal of the Science of Food and Agriculture·Luca ChiesaFrancesco Arioli
Sep 1, 2015·The Journal of Steroid Biochemistry and Molecular Biology·Nathalie De ClercqLynn Vanhaecke
Apr 16, 2016·Journal of Animal Physiology and Animal Nutrition·L ChiesaE Manuali
Mar 15, 2015·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·F ArioliL Chiesa
Nov 28, 2012·Steroids·Marco FidaniFrancesco Arioli
Jan 8, 2014·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Eva de RijkeLeendert A van Ginkel
Jun 17, 2016·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Luca ChiesaFrancesco Arioli
May 3, 2018·Food Additives & Contaminants. Part B, Surveillance·Vita GiacconeVincenzo Ferrantelli
Feb 1, 2019·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Danilo PitardiElena Bozzetta
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Jan 4, 2018·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Cassandra D PaulingVictoria L Jackson

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