Investigation on the stability of saquinavir loaded liposomes: implication on stealth, release characteristics and cytotoxicity

International Journal of Pharmaceutics
Lakshmi Narashimhan RamanaUma Maheswari Krishnan

Abstract

Although anti-retroviral therapy is the most efficient disease management strategy for HIV-AIDS, its applications are limited by several factors including the low bioavailability and first pass metabolism of the drugs. Nanocarriers such as liposomes have been developed to circumvent some of these problems. We report here preparation of novel liposome formulations for efficient delivery of anti-retroviral drugs to mammalian cells in culture. The liposomes were prepared and surface was modified using poly (ethylene glycol). Encapsulation efficiency of the anti-retroviral drug saquinavir was found to be approximately 33% and also exhibited sustained release of the drug. Although PEGylated liposomes were more stable in protein-supplemented media, had better colloidal stability and exhibited lesser sonochemical stability due to lower cavitation threshold. The cell viability studies using Jurkat T-cells revealed that the PEGylated liposomes loaded with saquinavir were less cytotoxic as compared to the non-PEGylated liposomes or free drug confirming the potential of the liposomes as a sustained drug-release system. The drug delivery potential of the liposomes loaded with Alexa flour 647 was evaluated using Jurkat T-cells and flow cyto...Continue Reading

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Citations

Oct 1, 2013·Drug Delivery and Translational Research·Shruti ChopraGuru V Betageri
Nov 25, 2014·Drug Delivery·Dhiraj BhavsarUma Maheswari Krishnan
Feb 26, 2014·Artificial Cells, Nanomedicine, and Biotechnology·Lalit KumarAmit Kumar Jain
Jan 10, 2016·The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases·Raimunda Sâmia Nogueira BrilhanteJosé Júlio Costa Sidrim
Dec 17, 2014·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Lakshmi Narashimhan RamanaUma Maheswari Krishnan
Oct 15, 2013·Biochimica Et Biophysica Acta·Lakshmi Narashimhan RamanaUma Maheswari Krishnan
Jul 16, 2013·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Mohammed ElmowafyMarjo Yliperttula
May 6, 2015·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Giovanna GiacaloneElias Fattal
Sep 30, 2014·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Aline Ferreira OuriqueRuy Carlos Ruver Beck
Aug 28, 2016·Nanomedicine : Nanotechnology, Biology, and Medicine·María José GómaraIsabel Haro
Feb 27, 2018·Frontiers in Pharmacology·Tabassum Khan, Pranav Gurav

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