Investigation the interaction between procyanidin dimer and α-glucosidase: Spectroscopic analyses and molecular docking simulation

International Journal of Biological Macromolecules
Taotao DaiChengmei Liu

Abstract

Procyanidins are reported to inhibit α-glucosidase, which may be a useful attribute for developing functional foods that control post-prandial blood sugar levels. At present, the nature of the molecular interaction between procyanidins and α-glucosidase is poorly understood. In this study, spectroscopic analyses and computer simulations were used to investigate the interactions between α-glucosidase and B-type procyanidin dimer (BPD). Our results suggest that BPD binds to α-glucosidase through a combination of hydrophobic and hydrophilic interactions. This hypothesis was based on measurements of the intrinsic fluorescence quenching, conformational changes, and surface hydrophobicity of the α-glucosidase after binding. Thermodynamic analysis suggested that α-glucosidase had one binding site for BPD and that the interaction was spontaneous. Homologous modeling of α-glucosidase was used to provide information about the precise nature of the molecular interactions. Molecular docking analysis suggested that BPD formed hydrogen bonds and hydrophobic interactions with α-glucosidase when it bound to its active site. This research offers new insights into the mechanism of interaction between procyanidins and α-glucosidase, which may be ...Continue Reading

Citations

Jan 27, 2021·Journal of Enzyme Inhibition and Medicinal Chemistry·Sonia FlorisBenedetta Era
Sep 12, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Uday HossainParames C Sil
May 19, 2021·Bioorganic Chemistry·Jianhua LvYu Li
Nov 19, 2021·Critical Reviews in Food Science and Nutrition·Yao ChenZhiyong He

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.