Investigations into Isoniazid Treated Mycobacterium tuberculosis by Electrospray Mass Spectrometry Reveals New Insights into Its Lipid Composition

Journal of Pathogens
Rahul PalZeeshan Fatima

Abstract

Many of the earlier studies involving the effect of isoniazid (INH) treatment have solely focused on the fatty acyl (FA) category of Mycobacterium tuberculosis (MTB) lipids. This motivated us with the major interest to examine the impact of INH on various other categories of MTB lipids. Towards this, we chose to interpret our mass spectral data (LC-ESI-MS) by a standalone software, MS-LAMP, in which "Mtb LipidDB" was integrated. Analysis by MS-LAMP revealed that INH treatment can alter the composition of "glycerolipids (GLs)" and "glycerophospholipids (GPLs)" categories of MTB lipids, in addition to the variations to FA category. Interpretation by "MycoMass" database yielded similar results as that of Mtb LipidDB, except that significant alterations to polyketides (PKs) category also were observed. Probing biosynthetic pathways of certain key lipids belonging to any of GLs, GPLs, and PKs categories can be attractive target(s) for drug discovery or can be useful to identify means to overcome drug resistance or to obtain insights into the causal factors of virulence. To the best of our knowledge, this is the first report hinting at the influence of INH on GLs, GPLs, and PKs of MTB.

References

Oct 29, 1999·European Journal of Biochemistry·K Athenstaedt, G Daum
Aug 8, 2002·Current Pharmaceutical Biotechnology·E K SchroederL A Basso
Nov 23, 2006·Biochemistry·Argyrides ArgyrouJohn S Blanchard
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Madhulika JainJeffery S Cox
Jun 29, 2007·Transactions of the Royal Society of Tropical Medicine and Hygiene·Julien ZwangStephen M Tollman
Aug 30, 2008·Glycobiology·Jeffrey S Schorey, Lindsay Sweet
Apr 7, 2010·The Journal of Biological Chemistry·Yasu S MoritaMalcolm J McConville
Jul 20, 2010·Annual Review of Analytical Chemistry·Stephen J Blanksby, Todd W Mitchell
Dec 27, 2011·Chemistry & Biology·Emilie LayreD Branch Moody
Mar 19, 2015·International Journal of Cancer. Journal International Du Cancer·Eyra MarienJohannes V Swinnen
Jan 21, 2016·International Journal of Mycobacteriology·Lingaraja JenaBhaskar C Harinath
Oct 19, 2016·The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases·Rahul PalZeeshan Fatima
Feb 7, 2017·Microbiological Research·Chunyan LiJianping Xie

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Software Mentioned

MycoMass
MS
LAMP
MassLynx

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