PMID: 16514181Mar 4, 2006Paper

Investigations of a human embryonic globin gene silencing element using YAC transgenic mice

Experimental Biology and Medicine
P A NavasG Stamatoyannopoulos

Abstract

A silencing element has been previously located upstream of the human epsilon-globin gene promoter using transient assays and transgenic mice carrying plasmid constructs in which the element has been deleted or its transcriptional motifs have been mutated. To investigate whether this element functions in the context of the whole beta-globin locus, we analyzed epsilon-globin gene expression in transgenic mice carrying a deletion of the silencing element in the context of a 213-kilobase human beta-globin yeast artificial chromosome (beta-YAC). epsilon-Globin gene expression was measured during embryonic and fetal development and in adult mice. epsilon-mRNA levels in embryonic cells in Day 12 blood were as high as those measured in wild-type beta-YAC controls, indicating that the deletion does not affect epsilon gene promoter function. epsilon-Globin gene expression was confined to the embryonic cells, indicating that deletion of this silencing element did not affect epsilon-globin developmental expression in the context of the beta-YAC. These results suggest that in the context of the whole beta-globin locus, other proximal and upstream epsilon gene promoter elements as well as competition by the downstream globin genes contribut...Continue Reading

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Citations

Apr 4, 2007·The Journal of Biological Chemistry·Xiaochun Long, Joseph M Miano
Dec 7, 2010·Developmental Biology·Maximilian Haeussler, Jean-Stéphane Joly
Feb 8, 2011·Blood Cells, Molecules & Diseases·Jeremy W RuponGordon D Ginder

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