Investigations on oxygen limitations of adherent cells growing on macroporous microcarriers.

Cytotechnology
A PreissmannW Noé

Abstract

Macroporous microcarriers are commonly applied to fixed and fluidized bed bioreactors for the cultivation of stringent adherent cells. Several investigations showed that these carriers are advantageous in respect to a large surface area (Griffiths, 1990; Looby, 1990a).When growing a rC-127 cell line on Cytoline 2 (Pharmacia Biotech), no satisfactory product yield could be achieved. A possible limitation in the supply of nutrient components was investigated to explain these poor results. No significant concentration gradients could be detected. Nevertheless, fluorescence staining revealed a decreasing viability, particularly inside the macroporous structure. Therefore, oxygen transfer to and into the carriers was examined by means of an oxygen microprobe during the entire process. Additional mathematical modeling supported these results.The maximum penetration depth of oxygen was determined to be 300 μm. A critical value influencing the oxygen uptake rate of the rC-127 cells occured at a dissolved oxygen concentration of 8% of air saturation. A significant mass transfer resistance within a laminar boundary film at the surface of the carrier could be detected. This boundary layer had a depth of 170 μm. The results showed that eve...Continue Reading

References

Aug 1, 1990·Trends in Biotechnology·D Looby, B Griffiths
Mar 1, 1992·Enzyme and Microbial Technology·T J Nikolai, W S Hu
Jul 1, 1986·Biotechnology and Bioengineering·R WittierK Schügerl
Aug 20, 1991·Biotechnology and Bioengineering·B A Westrin, A Axelsson

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Citations

Mar 27, 2018·Biotechnology and Bioengineering·Ashkan YekrangSafakarKidong Park
Feb 5, 2004·Biotechnology and Bioengineering·A Y YokomizoC A Pereira
Jul 17, 2015·Expert Review of Vaccines·Lilí Esmeralda Gallo-RamírezUdo Reichl

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