Investigations on the binding and antioxidant properties of the plant flavonoid fisetin in model biomembranes

FEBS Letters
Bidisa SenguptaPradeep K Sengupta

Abstract

Plant flavonoids are emerging as potent therapeutic drugs for free radical mediated diseases, for which cell membranes generally serve as targets for lipid peroxidation and related deleterious effects. Screening and characterization of these ubiquitous, therapeutically potent polyphenolic compounds, require a clear understanding regarding their incorporation and possible location in membranes, as well as quantitative estimates of their antioxidative and radical scavenging capacities. Here, we demonstrate the novel use of the intrinsic fluorescence characteristics of the plant flavonoid fisetin (3,3',4',7-OH flavone) to explore its binding and site(s) of solubilisation in egg lecithin liposomal membranes. Spectrophotometric assays have been used to obtain quantitative estimates of its antioxidative capacity. Furthermore, our quantum mechanical semi-empirical calculations provide a quantitative measure for the free radical scavenging activity of fisetin from the OH (at 3,3', 4', 7 positions of the molecule)-bond dissociation enthalpies. Implications of these findings are discussed.

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Citations

Nov 6, 2012·Antioxidants & Redox Signaling·Naghma KhanHasan Mukhtar
Jun 21, 2013·PloS One·Bidisha SenguptaPradeep K Sengupta
Sep 26, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Sik-Won ChoiSeong Hwan Kim
Jul 16, 2010·Immunopharmacology and Immunotoxicology·Tae-Yong Shin, Sang-Hyun Kim
Nov 28, 2012·Journal of Photochemistry and Photobiology. B, Biology·Biswapathik PahariPradeep K Sengupta
Jun 1, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ju Yeon KimMyeong Jin Nam
Nov 14, 2014·The Journal of Physical Chemistry. B·Bidisha SenguptaCari Hampton
Nov 17, 2009·Journal of Photochemistry and Photobiology. B, Biology·Sudip ChaudhuriPradeep K Sengupta
Jun 15, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Patrudu S Makena, King-Thom Chung
Oct 28, 2015·Journal of Photochemistry and Photobiology. B, Biology·Shrabanti DasPradipta Purkayastha
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Aug 2, 2005·Biochemical and Biophysical Research Communications·Bidisa Sengupta, Jan Swenson
Sep 13, 2005·Journal of Photochemistry and Photobiology. B, Biology·Sujit Kumar GhoshSubhash Chandra Bhattacharya
Jul 26, 2005·Journal of Photochemistry and Photobiology. B, Biology·Bidisa SenguptaPradeep K Sengupta
Nov 23, 2005·Biochemical and Biophysical Research Communications·Bidisa SenguptaJan Swenson
Dec 27, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Iwona Inkielewicz-StepniakMichal Wozniak
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Jan 29, 2008·Experimental Biology and Medicine·Sang-Hyun KimTae-Yong Shin

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