Investigations on the role of flagella in adhesion of Pseudomonas aeruginosa to mouse and human corneal epithelial proteins

Ophthalmic Research
L D Hazlett, X L Rudner

Abstract

Antiflagellar monoclonal antibodies (MAbs) were used in an overlay assay to determine whether flagella bind to blots of mouse and human corneal epithelial proteins (CEPs). The role of carbohydrates and surface charge was also explored by preincubation of blots with periodate or neuraminidase, or flagella with monosaccharides or charged compounds, respectively. Periodate slightly decreased binding of flagella, while sialic acid inhibited binding, and the effect was dose dependent. Neuraminidase treatment of blots or incubation of flagella with a negatively, but not a positively charged compound, also blocked binding. The data suggest that flagella interact with mouse and human CEPs by electrostatic mechanisms.

Citations

Apr 19, 2011·Applied and Environmental Microbiology·Victoria B TranClayton J Radke
Oct 16, 2004·Expert Review of Anti-infective Therapy·Sang Sun Yoon, Daniel J Hassett
Jan 1, 1996·Critical Reviews in Microbiology·S Moens, J Vanderleyden
Jun 7, 2013·Biomaterials Science·Olena RzhepishevskaMadeleine Ramstedt
Mar 13, 2002·Paediatric Drugs·Serina StrettonMark D P Willcox

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