Invited Review: Cryo-scanning electron microscopy (CSEM) in the advancement of functional plant biology. Morphological and anatomical applications

Functional Plant Biology : FPB
Margaret E McCullyCheng Huang

Abstract

Cryo-scanning electron microscopy (CSEM) is reviewed by exploring how the images obtained have changed paradigms of plant functions and interactions with their environment. Its power to arrest and stabilise plant parts in milliseconds, and to preserve them at full hydration for examination at micrometre resolution has changed many views of plant function. For example, it provides the only feasible way of accurately measuring stomatal aperture during active transpiration, and volume and shape changes in guard cells, or examining the contents of laticifers. It has revealed that many xylem conduits contain gas, not liquid, during the day, and that they can be refilled with sap and resume water transport. It has elucidated the management of ice to prevent cell damage in frost tolerant plants and has revealed for the first time inherent biological and physical features of root/soil interactions in the field. CSEM is increasingly used to reveal complementary structural information in studies of metabolism, fungal infection and symbiosis, molecular and genetic analysis.

References

May 1, 1994·Microscopy Research and Technique·C X HuangM E McCully
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·A M ChaudhuryW J Peacock
Mar 6, 1999·Current Opinion in Plant Biology·A M ChaudhuryW Peacock
Nov 26, 1999·The Plant Journal : for Cell and Molecular Biology·M RauscherH B Deising
Feb 2, 2000·Scanning·J Nijsse, A C van Aelst
Mar 9, 2000·Trends in Plant Science·M C HawesX Zhao
May 16, 2000·The Plant Cell·R A BurtonG B Fincher
Jul 4, 2001·Journal of Experimental Botany·W Van IeperenU Van Meeteren
Jul 4, 2001·Journal of Experimental Botany·E A GolovinaA C Van Aelst
Feb 19, 2002·Fungal Genetics and Biology : FG & B·Stefan G R WirselKurt W Mendgen
Jun 18, 2002·Plant Physiology·Joanne E BurnRichard E Williamson
Apr 26, 2003·Annals of Botany·Joost T Van DongenAdrianus C Borstlap
Jul 30, 2003·Journal of Experimental Botany·Melvin T Tyree, Herve Cochard
Dec 6, 2003·Plant Physiology·Hervé CochardCatherine Coutand
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Margaret E. McCully
Oct 23, 2004·Science·Jamie A PighinA Lacey Samuels
Feb 23, 2005·Journal of Experimental Botany·Leigh K GebbieRichard E Williamson
May 10, 2005·Eukaryotic Cell·M Gabriela RocaNick D Read
Oct 13, 2005·The New Phytologist·Philippe HinsingerWalter W Wenzel
Nov 10, 2005·Plant & Cell Physiology·Yingru WuElizabeth S Dennis
Mar 17, 2006·The New Phytologist·M J Canny, C X Huang
Oct 17, 2006·Plant Physiology·Stefan MayrSilvia B Kikuta
Nov 23, 2006·Plant Physiology·Peter J Franks, Graham D Farquhar
Mar 3, 2007·Annals of Botany·Edvaldo A A da SilvaHenk W M Hilhorst

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Citations

Mar 13, 2014·Journal of Experimental Botany·Hae Koo KimIldoo Hwang
Jun 1, 2012·Functional Plant Biology : FPB·Martin Canny
Nov 11, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Hans J EnsikatWilhelm Barthlott
Jul 5, 2012·Biomacromolecules·Petra B WelzelCarsten Werner

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