Involution dependent changes in distribution and localization of bax, survivin, caspase-3, and calpain-1 in the rat endometrium

Microscopy Research and Technique
Emel Alan, Narin Liman

Abstract

The endometrial layer of the uterus is characterized by continuous cycle of cell growth and apoptosis in response to hormonal changes. Apoptosis is regulated by several apoptotic regulators, but their significance in involuting uterus has not been well understood. For that reason, aim of this study was to investigate possible role of apoptosis-related proteins (bax and survivin) and enzymes (caspase-3 and calpain-1) in the involuting uterus of the rat, using immunohistochemistry. Our results indicated cytoplasmic and nuclear immunostaining for bax, caspase-3, calpain-1 and survivin proteins were found in the endometrial epithelium and stromal cells such as fibroblasts, mast cells and macrophages, and blood vessels; however, calpain-1 immunoreactivity in the endometrial fibroblast was quite weak or absent. Supranuclear punctate bax immunolabelling was also observed in the endometrial fibroblasts and luminal and glandular epithelial cells from days 1st and 3rd following parturition, respectively. Although survivin was localized in the apical cytoplasm underneath the apical membrane of the luminal epithelium on the 1st and 3rd days, it was also localized in the apicolateral membrane and basal cytoplasm on the 10th and 15th days of...Continue Reading

References

Mar 1, 1991·Journal of Cellular Physiology·J J JeffreyW T Roswit
Jan 1, 1984·Anatomy and Embryology·S S Craig, W P Jollie
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·Y T HsuR J Youle
Aug 1, 1997·Nature Medicine·G AmbrosiniD C Altieri
Jan 7, 1998·The Journal of Cell Biology·K G WolterR J Youle
Jun 13, 1998·The Journal of Biological Chemistry·M ZhouC M Bitler
Jun 27, 1998·Biochemical and Biophysical Research Communications·E Carafoli, M Molinari
Jan 28, 1999·Biochemical and Biophysical Research Communications·B ShiJ Mahajna
Apr 25, 2000·Cell Death and Differentiation·R HoetelmansJ H Dierendonck
May 29, 2000·Molecular Human Reproduction·R KonnoA Yajima
Jan 17, 2002·Medical Electron Microscopy : Official Journal of the Clinical Electron Microscopy Society of Japan·Y Otsuki
Mar 19, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Allen M Gown, Mark C Willingham
Apr 12, 2002·Acta Obstetricia Et Gynecologica Scandinavica·Rainer LehnerKenneth R Shroyer
Jul 5, 2002·The Biochemical Journal·Michela Capano, Martin Crompton
Aug 13, 2002·Nature Medicine·Olivier P Blanc-BrudeDario C Altieri
Aug 14, 2002·Life Sciences·Valery F ThompsonDarrel E Goll
Jul 5, 2003·Physiological Reviews·Darrell E GollJinyang Cong
Oct 29, 2003·Theriogenology·S Van CruchtenP Simoens
Oct 29, 2003·Molecular and Cellular Endocrinology·C Adriana Mendoza-RodríguezMarco A Cerbón
Jan 27, 2005·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·J-P LeeH-S Ryu
Feb 3, 2005·Molecular Reproduction and Development·Peng WeiYi-Xun Liu
Feb 24, 2005·Cancer Biology & Therapy·Zhaoyu Jin, Wafik S El-Deiry
May 6, 2005·Journal of Vascular Surgery·Hector F SimosaMichael S Conte
May 30, 2006·Molecular Cancer Therapeutics·Seiji Fukuda, Louis M Pelus
Jul 28, 2006·Molecular Human Reproduction·Hideaki AbeYoshinori Otsuki
Oct 21, 2006·Cell Death and Differentiation·M LamkanfiP Vandenabeele
Jan 27, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Olivier P Blanc-BrudeAlain Tedgui

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis