Involvement in Denitrification is Beneficial to the Biofilm Lifestyle of Comamonas testosteroni: A Mechanistic Study and Its Environmental Implications

Environmental Science & Technology
Yichao WuBin Cao

Abstract

Comamonas is one of the most abundant microorganisms in biofilm communities driving wastewater treatment. Little has been known about the role of this group of organisms and their biofilm mode of life. In this study, using Comamonas testosteroni as a model organism, we demonstrated the involvement of Comamonas biofilms in denitrification under bulk aerobic conditions and elucidated the influence of nitrate respiration on its biofilm lifestyle. Our results showed that C. testosteroni could use nitrate as the sole electron acceptor for anaerobic growth. Under bulk aerobic condition, biofilms of C. testosteroni were capable of reducing nitrate, and intriguingly, nitrate reduction significantly enhanced viability of the biofilm-cells and reduced cell detachment from the biofilms. Nitrate respiration was further shown to play an essential role in maintaining high cell viability in the biofilms. RNA-seq analysis, quantitative polymerase chain reaction, and liquid chromatography-mass spectrometry revealed a higher level of bis(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) in cells respiring on nitrate than those grown aerobically (1.3 × 10(-4) fmol/cell vs 7.9 × 10(-6) fmol/cell; P < 0.01). C-di-GMP is one universal signali...Continue Reading

References

Oct 6, 1998·Applied and Environmental Microbiology·K D XuG A McFeters
Jun 1, 2000·Microbial Ecology·D PatureauR Moletta
Aug 12, 2003·Nature Structural Biology·Michela G BerteroNatalie C J Strynadka
Dec 10, 2003·Current Opinion in Microbiology·Jeremy S WebbStaffan Kjelleberg
Jun 29, 2006·Annals of the New York Academy of Sciences·Ripudaman SinghSuresh I S Rattan
Jul 22, 2006·Trends in Microbiology·Rajbir SinghRakesh K Jain
Aug 10, 2006·Annual Review of Genetics·Urs Jenal, Jacob Malone
Aug 29, 2006·Applied and Environmental Microbiology·Tracy K TealDianne K Newman
Oct 24, 2006·FEMS Microbiology Letters·Morten GjermansenTim Tolker-Nielsen
Aug 21, 2007·Applied and Environmental Microbiology·Michael KühlRoland Thar
Nov 13, 2007·Journal of Bacteriology·Alan J Wolfe, Karen L Visick
Feb 12, 2008·Nature Reviews. Microbiology·Philip S Stewart, Michael J Franklin
Apr 22, 2008·FEMS Microbiology Letters·Sofia AnderssonGunnel Dalhammar
Nov 1, 2008·Environmental Microbiology·Mike S M Jetten
Mar 17, 2009·Nature Reviews. Microbiology·Regine Hengge
Sep 8, 2009·Applied and Environmental Microbiology·Ying-Fei MaShuang-Jiang Liu
Jan 28, 2010·Environmental Science & Technology·Yu-Chuan JuangJuin-Yih Lai
Aug 3, 2010·Nature Reviews. Microbiology·Hans-Curt Flemming, Jost Wingender
May 17, 2011·Journal of Bacteriology·Renee M SavilleAlfred M Spormann
Aug 29, 2012·Environmental Microbiology·Bin CaoHaluk Beyenal
Apr 17, 2014·MBio·Aimee K WesselMarvin Whiteley
Jul 1, 2014·Environmental Microbiology·Gianlucca G NicastroRegina L Baldini

❮ Previous
Next ❯

Citations

Jan 19, 2016·Frontiers in Microbiology·Qi YeJing Zhang
May 21, 2019·Microbial Biotechnology·Caroline Van der HeydenGeertui Rasschaert

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.