Involvement in surface antigen expression by a moonlighting FG-repeat nucleoporin in trypanosomes

Molecular Biology of the Cell
Jennifer M HoldenMark C Field

Abstract

Components of the nuclear periphery coordinate a multitude of activities, including macromolecular transport, cell-cycle progression, and chromatin organization. Nuclear pore complexes (NPCs) mediate nucleocytoplasmic transport, mRNA processing, and transcriptional regulation, and NPC components can define regions of high transcriptional activity in some organisms at the nuclear periphery and nucleoplasm. Lineage-specific features underpin several core nuclear functions and in trypanosomatids, which branched very early from other eukaryotes, unique protein components constitute the lamina, kinetochores, and parts of the NPCs. Here we describe a phenylalanine-glycine (FG)-repeat nucleoporin, TbNup53b, that has dual localizations within the nucleoplasm and NPC. In addition to association with nucleoporins, TbNup53b interacts with a known trans-splicing component, TSR1, and has a role in controlling expression of surface proteins including the nucleolar periphery-located, procyclin genes. Significantly, while several nucleoporins are implicated in intranuclear transcriptional regulation in metazoa, TbNup53b appears orthologous to components of the yeast/human Nup49/Nup58 complex, for which no transcriptional functions are known. T...Continue Reading

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Citations

Apr 20, 2019·F1000Research·Mark C Field, Michael P Rout

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Methods Mentioned

BETA
confocal microscopy
three-hybrid
pull down
pullout
RNA-seq
RNAseq
immunoprecipitation
PCR
transfection

Software Mentioned

kstest2
MATLAB
ImageJ
Huygens
HMMER
ProFound
BLAST
Adobe Photoshop
MiniOpticon
Fiji

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