Involvement of 67-kDa laminin receptor-mediated myosin phosphatase activation in antiproliferative effect of epigallocatechin-3-O-gallate at a physiological concentration on Caco-2 colon cancer cells

Biochemical and Biophysical Research Communications
Daisuke UmedaHirofumi Tachibana

Abstract

Previously we reported that 67-kDa laminin receptor (67LR) mediates epigallocatechin-3-O-gallate (EGCG)-induced cell growth inhibition and reduction of myosin regulatory light chain (MRLC) phosphorylation at Thr-18/Ser-19, which is important for cytokinesis. Here, we found that human colon adenocarcinoma Caco-2 cells exhibited higher expression level of 67LR and EGCG at a physiologically achievable concentration (1 microM) significantly accumulated the cells in G(2)/M phase without affecting expression of Wnt-signaling components. We also found that myosin phosphatase targeting subunit 1 (MYPT1) phosphorylation at Thr-696, which inhibits myosin phosphatase and promotes MRLC phosphorylation, was reduced in response to 1 microM EGCG. 67LR knockdown by RNA interference abolished the inhibitory effects of 1 microM EGCG on cell cycle progression and the phosphorylation of MRLC and MYPT1. These results suggest that through 67LR, EGCG at a physiological concentration can activate myosin phosphatase by reducing MYPT1 phosphorylation and that may be involved in EGCG-induced cell growth inhibition.

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Citations

Jun 6, 2012·PloS One·Yoshinori FujimuraHirofumi Tachibana
Mar 23, 2011·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Hirofumi Tachibana
Sep 4, 2012·Biotechnology Advances·Isis S SantosEliana B Souto
Oct 6, 2010·Canadian Journal of Physiology and Pharmacology·Xueying YangWenjun Shi
Jan 31, 2013·Journal of Tissue Engineering and Regenerative Medicine·Georges SabraPatrick Vermette
Mar 20, 2012·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xueying YangWenjun Shi
Nov 3, 2009·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xueying YangDali Tian
Feb 11, 2015·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xueying YangJibin Lu
Sep 21, 2016·Critical Reviews in Food Science and Nutrition·Ren-You GanHarold Corke
Dec 14, 2016·Phytotherapy Research : PTR·Christine M KaufmannMichael W Pfaffl
Mar 2, 2012·Food & Function·Akira Murakami, Kohta Ohnishi
Jan 30, 2015·Biological Reviews of the Cambridge Philosophical Society·Vincent DiGiacomo, Daniel Meruelo

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