PMID: 11607301Jun 15, 1992Paper

Involvement of a chloroplast HSP70 heat shock protein in the integration of a protein (light-harvesting complex protein precursor) into the thylakoid membrane

Proceedings of the National Academy of Sciences of the United States of America
S YalovskyR Nechushtai

Abstract

Molecular chaperones, including those belonging to the 70-kDa family of heat shock proteins (HSP70), assist both the translocation of proteins across membranes and their assembly into oligomeric complexes. We purified a chloroplast HSP70 (ct-HSP70) and demonstrated that it plays a major role in the insertion of the precursor of the major light-harvesting complex of photosystem II (pLHCP; an integral membrane protein) into the thylakoids (the inner membranes of the chloroplast). Addition of the purified ct-HSP70 is necessary for efficient insertion of pLHCP into isolated thylakoid membranes. This activity of the purified ct-HSP70 is similar to that previously reported for the total stromal extract. When the chloroplast stromal extract is depleted of HSP70, a correlative reduction in the insertion activity of pLHCP is observed. The interaction between the ct-HSP70 and pLHCP involves physical association. The purified HSP70 acts directly on the membrane protein, presumably prevents its refolding, and thereby helps to maintain its competence for insertion into membranes.

References

Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·D R PallerosA L Fink
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D Amir-ShapiraN Brot
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J S MarshallE Vierling
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G LiuL L Randall
Jul 5, 1987·Nature·J Ellis
Nov 16, 1990·Science·R J Ellis

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Citations

May 1, 1995·Photosynthesis Research·R NechushtaiP R Chitnis
Oct 1, 1996·Plant Molecular Biology·R S BostonE Vierling
Feb 8, 1994·Biochimica Et Biophysica Acta·S Jansson
Aug 29, 2003·The Plant Journal : for Cell and Molecular Biology·Dongsun CaoChi-Lien Cheng
Feb 15, 2000·European Journal of Biochemistry·K KosemundH Paulsen
Nov 1, 1996·Biological Reviews of the Cambridge Philosophical Society·A BakerM R Pool
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Danny J. Schnell
Dec 17, 2008·The New Phytologist·Paul Jarvis
Sep 4, 2007·Protein Science : a Publication of the Protein Society·Addmore ShonhaiGregory L Blatch
Aug 1, 1993·European Journal of Biochemistry·H PaulsenN Kühlein
May 26, 2010·The Journal of Biological Chemistry·Sebastian Falk, Irmgard Sinning
Dec 24, 2017·Essays in Biochemistry·Robert G SowdenPaul Jarvis
Jan 31, 1997·The Journal of Biological Chemistry·L Kourtz, K Ko
Apr 27, 1999·Biochimica Et Biophysica Acta·F A WollmanR Nechushtai

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