Involvement of a gelsolin-related protein in spermatogenesis of the earthworm Lumbricus terrestris

Cell and Tissue Research
Evelyn KrügerJochen D'Haese

Abstract

A gelsolin-related protein was isolated from seminal vesicles of the annelid Lumbricus terrestris. Compared with the isoforms of the gelsolin-related protein previously found in the muscle of the annelid body wall, the isolated protein was assigned to the first isoform (EWAM-P1) because of its electrophoretic mobility, chromatographic elution behaviour, immunological cross-reactivity and identical nucleotide sequence of segments obtained by reverse transcription/polymerase chain reaction. Immunofluorescence studies with smear preparations of developing male germ cells revealed characteristic changes of the local distribution of actin and EWAM-P1 during spermatogenesis. These changes were correlated with the developmental transport processes and structural alterations. F-actin, as revealed by rhodamine-phalloidin staining, formed a toroid-shaped structure in cytoplasmic bridges connecting the germ cells to a central cytophore during the developmental stages. An actin antibody reacting with both G- and F-actin demonstrated that actin was concentrated at the proximal and distal parts of the spermatocytes. EWAM-P1 was also localized in these regions, with intense staining in the distal part of spermatocytes and young spermatids in ...Continue Reading

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Citations

Oct 25, 2012·Environmental Monitoring and Assessment·Beryl Vedha YesudhasonSudhakar Sivasubramaniam
Feb 5, 2013·Zoology : Analysis of Complex Systems, ZACS·Anna Z Urbisz, Piotr Świątek
Aug 28, 2012·Micron : the International Research and Review Journal for Microscopy·Raja Ben AhmedPiotr Świątek
May 28, 2013·Archives of Biochemistry and Biophysics·Andreas UngerHorst Hinssen
May 22, 2013·Expert Opinion on Drug Metabolism & Toxicology·Margaret Clotworthy, Kathy Archibald

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