Involvement of a mate chaperone (TorD) in the maturation pathway of molybdoenzyme TorA.

The Journal of Biological Chemistry
Marianne IlbertChantal Iobbi-Nivol

Abstract

As many prokaryotic molybdoenzymes, the trimethylamine oxide reductase (TorA) of Escherichia coli requires the insertion of a bis(molybdopterin guanine dinucleotide)molybdenum cofactor in its catalytic site to be active and translocated to the periplasm. We show in vitro that the purified apo form of TorA was activated weakly when an appropriate bis(molybdopterin guanine dinucleotide)molybdenum source was provided, whereas addition of the TorD chaperone increased apoTorA activation up to 4-fold, allowing maturation of most of the apoprotein. We demonstrate that TorD alone is sufficient for the efficient activation of apoTorA by performing a minimal in vitro assay containing only the components for the cofactor synthesis, apoTorA and TorD. Interestingly, incubation of apoTorA with TorD before cofactor addition led to a significant increase of apoTorA activation, suggesting that TorD acts on apoTorA before cofactor insertion. This result is consistent with the fact that TorD binds to apoTorA and probably modifies its conformation in the absence of cofactor. Therefore, we propose that TorD is involved in the first step of TorA maturation to make it competent to receive the cofactor.

References

Aug 1, 1982·Journal of Bacteriology·V Stewart, C H MacGregor
Mar 1, 1994·Molecular Microbiology·V MéjeanM C Pascal
Oct 18, 1996·Journal of Molecular Biology·F SchneiderJ Knäblein
Apr 20, 2001·Molecular Microbiology·I J OresnikR J Turner
May 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·M W Ribbe, B K Burgess
May 19, 2001·Archives of Microbiology·A HasonaK T Shanmugam
Sep 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·S GonV Méjean
Nov 7, 1996·Chemical Reviews·Russ Hille
Aug 23, 2002·Protein Science : a Publication of the Protein Society·Samuel TranierJean-Pierre Samama

❮ Previous
Next ❯

Citations

Nov 19, 2004·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Sep 29, 2012·The Journal of Biological Chemistry·Kang-Mu LeeSang Sun Yoon
Jul 25, 2006·Infection and Immunity·Dirk HofreuterJorge E Galán
Oct 4, 2011·Journal of Bacteriology·David RedelbergerChantal Iobbi-Nivol
Sep 13, 2005·Annual Review of Microbiology·Mechthild PohlschröderAlex Haddad
Jun 16, 2004·Proceedings of the National Academy of Sciences of the United States of America·Mireille AnsaldiVincent Méjean
Jun 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Kostas HatzixanthisFrank Sargent
Dec 15, 2010·Biochemistry Research International·Meina Neumann, Silke Leimkühler
Nov 12, 2013·FEBS Letters·David RedelbergerChantal Iobbi-Nivol
Oct 16, 2015·FEMS Microbiology Reviews·Silke Leimkühler, Chantal Iobbi-Nivol
Oct 16, 2007·Journal of Molecular Biology·Eva-Maria Strauch, George Georgiou
Sep 24, 2004·The EMBO Journal·Rachael L JackFrank Sargent
Sep 14, 2007·Molecular Microbiology·Mireille AnsaldiVincent Méjean
Dec 13, 2005·Biochemical and Biophysical Research Communications·Olivier GenestChantal Iobbi-Nivol
Feb 10, 2004·Biochemical and Biophysical Research Communications·Kwabena J SarfoRaymond J Turner
Dec 4, 2012·Biochimica Et Biophysica Acta·Chantal Iobbi-Nivol, Silke Leimkühler
Dec 1, 2011·Biomolecular Concepts·Bo Hou, Thomas Brüser
Dec 4, 2010·Biochimica Et Biophysica Acta·Colin RobinsonSharon Mendel
Feb 9, 2010·Biochimica Et Biophysica Acta·Claire E Price, Arnold J M Driessen
Nov 17, 2006·FEMS Microbiology Letters·Olivier GenestChantal Iobbi-Nivol
Jun 13, 2009·FEMS Microbiology Letters·Olivier GenestChantal Iobbi-Nivol
Jun 12, 2016·Research in Microbiology·Olivier N LemaireChantal Iobbi-Nivol
Sep 26, 2006·Archives of Biochemistry and Biophysics·Tara L WinstoneRaymond J Turner
Apr 1, 2015·Journal of Bacteriology·Manuela Roggiani, Mark Goulian
Feb 23, 2005·The Journal of Biological Chemistry·Olivier GenestChantal Iobbi-Nivol
Oct 21, 2005·The Journal of Biological Chemistry·Marianne GuiralMarie-Thérèse Giudici-Orticoni
Oct 22, 2013·Chemical Society Reviews·Courtney Sparacino-WatkinsPartha Basu
Oct 25, 2007·Biochemical Society Transactions·F Sargent
Apr 7, 2006·The Journal of Biological Chemistry·Meina NeumannSilke Leimkühler
Jun 24, 2004·Canadian Journal of Microbiology·Raymond J TurnerFrank Sargent
Mar 30, 2013·The Journal of Biological Chemistry·Phillip RingelTobias Kruse
Jun 5, 2008·The Journal of Biological Chemistry·Olivier GenestChantal Iobbi-Nivol
Jan 26, 2005·Biochemical Society Transactions·K HatzixanthisF Sargent
Jun 28, 2018·Current Genetics·Jeffrey N Carey, Mark Goulian
Mar 8, 2017·Frontiers in Microbiology·Ilya V KublanovElizaveta A Bonch-Osmolovskaya

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.