PMID: 9181696Jun 15, 1997Paper

Involvement of a sperm protein sensitive to sulfhydryl-depleting reagents in mouse sperm-egg fusion

The Journal of Experimental Zoology
A MammotoA Miyake

Abstract

Effects of various sulfhydryl (SH)-depleting reagents on sperm-egg fusion were demonstrated. When sperm were treated with three plasma membrane-permeable SH-depleting reagents, N-ethyl-maleimide, sodium tetrathionate and 5,5'-dithiobis (2-nitro-benzoic acid), the rates of cleavage of eggs were significantly lower than those with control sperm. Neither the motility, penetration of the zona pellucida, acrosomal status of the sperm, nor sperm-egg binding was affected by the SH-depleting reagents. Fusion of sperm and zona-free egg was estimated by the sperm nuclear incorporation into the eggs, intracellular calcium mobilization, and cortical granule exocytosis in the eggs. Sperm-egg fusion was blocked dose-dependently when sperm were exposed to membrane-permeable SH-depleting reagents, but was not blocked by a membrane-impermeable SH-depleting reagent, eosin-5-maleimide. Blockage of fusion by sodium tetrathionate was completely reversed by an SH-reductant, dithiothreitol. These results suggest that a protein which is sensitive to SH-depleting reagents may play an important role in mouse sperm-egg fusion and that the functional SH region of the protein may be located at an intracellular site.

References

Jan 1, 1976·Methods in Cell Biology·H I Calvin
Sep 1, 1990·American Journal of Reproductive Immunology : AJRI·T MoriT Mori
Nov 1, 1984·Journal of Reproduction and Fertility·P S CuasnicúJ A Blaquier
Nov 30, 1993·Biochemical and Biophysical Research Communications·N MasumotoO Tanizawa
Oct 1, 1993·Molecular Reproduction and Development·J TaoJ K Critser
Dec 1, 1996·The Journal of Cell Biology·N MasumotoA Miyake

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Citations

Nov 8, 2011·Annual Review of Physiology·Janice P Evans
Jan 24, 2006·Molecular and Cellular Endocrinology·Joël R Drevet
Dec 18, 2001·Molecular Reproduction and Development·S ChatterjeeC Gagnon
Oct 13, 2017·Biology of Reproduction·Cristian O'Flaherty, David Matsushita-Fournier
Mar 30, 2018·Cellular and Molecular Life Sciences : CMLS·Birke J BenedikterFrank R M Stassen

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