Involvement of accumulated endogenous NOS inhibitors and decreased NOS activity in the impaired neurogenic relaxation of the rabbit proximal urethra with ischaemia

British Journal of Pharmacology
Hitoshi MasudaHiroshi Azuma

Abstract

1. We examined the effect of ischaemia on the neurogenic and nitric oxide (NO)-mediated urethral relaxation. 2. Rabbits were divided into control and urethral ischaemia (UI) groups, which was prepared by the partial occlusion of bilateral iliac arteries using blood vessel occluders. 3. Neurogenic and NO-mediated proximal urethral relaxation induced by electrical field stimulation (EFS) was greatly impaired in the UI group, while relaxation by sodium nitroprusside (SNP) as a NO donor showed no difference between the two groups. Pretreatment with L-arginine significantly improved but did not normalize the impaired relaxation in the UI group. Not only basal level, but also stimulated production of cyclic GMP with EFS, were significantly decreased in the UI group. 4. The tissue contents of N(G)-methyl-L-arginine (L-NMA) and asymmetric N(G), N(G)-dimethyl-L-arginine (ADMA) in the proximal urethra were increased following ischaemia. While L-arginine and symmetric N(G), N'(G)-dimethyl-L-arginine (SDMA) contents remained unchanged. Exogenously applied authentic L-NMA and ADMA (1 -- 100 microM) concentration-dependently inhibited the EFS-induced urethral relaxation in the control group. The inhibition with L-NMA and ADMA was undetectabl...Continue Reading

References

Jan 1, 1992·Journal of Cardiovascular Pharmacology·P VallanceS Moncada
Jan 1, 1991·Annual Review of Biochemistry·M Chinkers, D L Garbers
Jul 1, 1990·The Biochemical Journal·R G KnowlesS Moncada
Jan 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·H H SchmidtF Murad
Jan 1, 1992·The Journal of Urology·K E AnderssonA Tøttrup
Apr 1, 1991·Kidney International·T F LüscherD Diederich
Jan 1, 1990·Annual Review of Pharmacology and Toxicology·L J Ignarro
Jan 1, 1990·Japanese Journal of Pharmacology·Y Kobayashi, K Hattori
Sep 1, 1984·The Journal of Urology·S V Yalla, G L Andriole
Jan 1, 1983·The Journal of Urology·J G Blaivas, G A Barbalias
Sep 1, 1995·The American Journal of Physiology·R G BogleP Vallance
Jun 1, 1995·The Journal of Urology·B C BennettW C de Groat
Dec 1, 1995·British Journal of Urology·J LeveckisJ B Anderson
Feb 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·M A MoroS Moncada
Jun 1, 1996·British Journal of Pharmacology·A García-PascualD Triguero
Feb 15, 2001·The Journal of Urology·R KozlowskiK M Azadzoi

❮ Previous
Next ❯

Citations

May 18, 2006·Basic Research in Cardiology·X ChenC P Tiefenbacher
Sep 9, 2004·Current Opinion in Obstetrics & Gynecology·Mat H HoOmid Khorram
Sep 10, 2013·Progrès en urologie : journal de l'Association française d'urologie et de la Société française d'urologie·X GaméB Malavaud
Apr 22, 2006·BJU International·Viktoria WerkströmPetter Hedlund
Nov 25, 2003·The Journal of Urology·Tomonori YamanishiRussell Chess-Williams
Mar 24, 2017·International Journal of Urology : Official Journal of the Japanese Urological Association·Yukihito Higashi
Feb 14, 2008·International Journal of Urology : Official Journal of the Japanese Urological Association·Hitoshi Masuda
Feb 1, 2006·Vascular Medicine·Shelagh AnthonyPatrick Vallance
Nov 15, 2017·Nature Reviews. Urology·Fabiola Z Mónica, Edson Antunes
Jul 2, 2002·The Journal of Biological Chemistry·Arturo J Cardounel, Jay L Zweier
May 16, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Hitoshi MasudaHiroshi Azuma
Feb 28, 2002·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Hitoshi Masuda, Hiroshi Azuma
Mar 20, 2004·Journal of Agricultural and Food Chemistry·Ho Jin HeoDong-Hoon Shin
Dec 11, 2003·Atherosclerosis. Supplements·Cam T L TranPatrick Vallance

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.