PMID: 9525582Apr 3, 1998Paper

Involvement of actin microfilaments in the replication of human parainfluenza virus type 3

Journal of Virology
S GuptaA K Banerjee

Abstract

Several studies indicate that paramyxoviruses require a specific cellular factor(s) for transcription of their genomic RNAs. We previously reported that the cellular cytoskeletal protein actin, in its polymeric form, participates in the transcription of human parainfluenza virus type 3 (HPIV3) in vitro. In the present study, we investigated the role of the polymeric form of actin, i.e., the actin microfilaments of the cytoskeletal framework, in the reproduction of HPIV3 in vivo. Pulse-chase labeling analyses indicate that the viral nucleocapsid-associated proteins, NP and P, are present predominantly in the cytoskeletal framework during infection. By in situ hybridization, we found that viral mRNAs and genomic RNA were synthesized from the nucleocapsids that were bound to the cytoskeletal framework. Double immunofluorescent labeling and confocal microscopy of the cytoarchitecture revealed that the viral nucleocapsids are specifically localized on the actin microfilaments. Treatment of cells with the actin-depolymerizing agent, cytochalasin D, resulted in the inhibition of viral RNA synthesis and ribonucleoprotein accumulation. These results strongly suggest that actin microfilaments play an important role in the replication of ...Continue Reading

References

Aug 1, 1979·The Journal of Experimental Medicine·J A Griffin, R W Compans
Nov 1, 1977·The Journal of Cell Biology·C H DamskyL Warren
May 1, 1979·The Journal of Cell Biology·D L Tyrrell, A Ehrnst
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1991·Pharmacology & Therapeutics·A K BanerjeeB P De
Sep 13, 1991·Science·C L Sundell, R H Singer
Apr 1, 1990·The Journal of General Virology·S A MoyerS M Horikami
Jan 16, 1985·Biochemical and Biophysical Research Communications·B P De, A K Banerjee
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S A MoyerJ L Lessard
Jan 1, 1986·Annual Review of Biochemistry·T D Pollard, J A Cooper
Mar 1, 1987·Microbiological Reviews·A K Banerjee
Jun 1, 1971·The Journal of Cell Biology·B S SpoonerN K Wessells
Jun 1, 1980·Journal of Virology·N Genty, F Bussereau
Jan 15, 1983·Virology·K C StallcupB N Fields
Mar 1, 1981·The Journal of Cell Biology·S S Brown, J A Spudich
Jul 1, 1981·The Journal of Cell Biology·M Schliwa, J van Blerkom
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·B P DeA K Banerjee
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·H SasakiY Nonomura
May 1, 1994·Journal of Virology·R Pearce-PrattD M Phillips
Dec 7, 1995·Nature·S CudmoreM Way

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Citations

Jul 27, 2002·Annual Review of Cell and Developmental Biology·Gregory A Smith, Lynn W Enquist
Sep 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Santanu BoseAmiya K Banerjee
Sep 19, 2003·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Santanu Bose, Amiya K Banerjee
Nov 19, 2003·FEBS Letters·Shuiyun LanZhenghong Yuan
May 11, 2004·The Journal of Veterinary Medical Science·Hideo KatayamaChieko Kai
Feb 14, 2006·Cellular Microbiology·Kerstin RadtkeBeate Sodeik
Dec 10, 1999·The Journal of Biological Chemistry·T OginoK Mizumoto
Oct 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·P Mas, R N Beachy
Jun 7, 2008·PLoS Pathogens·Megan L ShawPeter Palese
Dec 28, 2004·Nature Medicine·Vira BitkoSailen Barik
Oct 1, 2007·Therapeutic Advances in Respiratory Disease·Lynette B FernandesRoy G Goldie
Nov 24, 2006·Journal of Virology·Tadasuke NaitoKyosuke Nagata
Nov 12, 2002·The Journal of Biological Chemistry·Mingjuan LiuBret A Hassel
Jul 11, 2001·Biochemical and Biophysical Research Communications·T OginoK Mizumoto
Nov 2, 1999·Virology·T Ohkawa, L E Volkman
Nov 1, 2003·Biochemical and Biophysical Research Communications·Tomoaki OginoKiyohisa Mizumoto
Nov 30, 2004·Virus Research·Toru Takimoto, Allen Portner
Jun 22, 2011·Journal of Proteome Research·Jae-Min SongSeung Il Kim
Aug 20, 2021·BioMed Research International·Chaoliang ZhangGuangyuan Zhang
Dec 22, 2005·Virology Journal·Ziying Han, Ronald N Harty

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