Involvement of Ad4BP/SF-1, DAX-1, and COUP-TFII transcription factor on steroid production and luteinization in ovarian theca cells.

Molecular and Cellular Biochemistry
Chiaki MurayamaTakashi Shimizu

Abstract

To examine the essential mechanisms of steroid production in ovarian theca cells, we analyzed the expression of genes associated with steroid production using simple culture system with serum medium. In addition, we examined the involvement of DAX-1, COUP-TFII, and Ad4BP/SF-1 transcription factors on the steroid production in theca cells. Theca cells begin to display an elongated or fibroblastic aspect within 24 h of culture. Over the next 48 h, they metamorphosed from the fibroblastic to the epitheloid phenotype. The number of theca cells increased during culture period. Androstenedione and progesterone production per cell decreased at 48-96 h compared with 0-48 h of culture. Steroidogenic acute regulatory protein (StAR) and CYP 17 genes expression decreased at 48 h compared with 0 h of culture, and afterward maintained a low level. In contrast, expression of 3beta-HSD and P450scc mRNAs increased at 48 h compared with 0 h of culture. Protein expression of Ab4BP/SF-1 maintained a constant level during culture. COUP-TFII protein expression showed a peak level at 24 h of culture period. DAX-1 protein expression began to increase at 48 h of culture. Our data suggested that the inhibition in CYP 17 and StAR genes by DAX-1 transcrip...Continue Reading

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Citations

Feb 21, 2013·Reproductive Biology and Endocrinology : RB&E·Boqun XuJiayin Liu
Jun 6, 2014·Biology of Reproduction·Raifish E Mendoza-VillarroelJacques J Tremblay
Jan 28, 2012·Reproduction, Fertility, and Development·Adriana VerduzcoBruce D Murphy
Jan 9, 2014·Journal of Peptide Science : an Official Publication of the European Peptide Society·T ShimizuK Fukuda
Feb 15, 2013·PloS One·Katja HummitzschRaymond J Rodgers
Dec 30, 2016·Birth Defects Research. Part C, Embryo Today : Reviews·Wassim Eid, Anna Biason-Lauber
Dec 9, 2020·Molecular Aspects of Medicine·Camilla H K Hughes, Bruce D Murphy
May 16, 2015·Biochemical and Biophysical Research Communications·Chiaki MurayamaTakashi Shimizu
Jul 21, 2009·Molecular and Cellular Endocrinology·Erling A HoivikMarit Bakke
Jun 15, 2010·Best Practice & Research. Clinical Endocrinology & Metabolism·Anna Biason-Lauber

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