Involvement of Aif1 in apoptosis triggered by lack of Hxk2 in the yeast Saccharomyces cerevisiae

FEMS Yeast Research
Loredana AmigoniSonia Colombo

Abstract

We recently showed that in hxk2Δ cells, showing constitutive localization of active Ras at the mitochondria, addition of acetic acid caused an increase of both apoptotic and necrotic cells compared with the wild-type strain, providing a new role for hexokinase 2 (EC 2.7.1.1) as an anti-apoptotic factor, besides its known role as a glycolytic enzyme and as a regulator of gene transcription of several Mig1-regulated genes. We also demonstrated that apoptosis induced by lack of Hxk2 may not require the activation of Yca1. Here, we show that deletion of HXK2 causes hypersensitivity to H2O2 and that addition of this well-known apoptotic stimulus to hxk2Δ cells causes an increase in the level ROS, apoptosis and mitochondrial membrane potential. We also show that deletion of AIF1 in hxk2Δ cells enhances survival after induction of apoptosis with both H2O2 and acetic acid, rescues the reduction of both growth rate and cell size, abrogates both H2O2 and acetic acid-induced ROS accumulation and decreases cell death, suggesting that Aif1 might be involved in both H2O2 and acetic acid-induced cell death in hxk2Δ cells. Moreover, we show that active Ras proteins relocalize to the plasma membrane and to the nucleus in hxk2Δ aif1Δ cells.

References

Jan 1, 1983·Methods in Enzymology·R J Rothstein
Nov 20, 1998·FEBS Letters·M LigrD H Wolf
May 20, 1999·The Journal of Cell Biology·F MadeoK U Fröhlich
Sep 28, 2002·Nature·Jeffrey A KleinSusan L Ackerman
Aug 3, 2004·Science·Douglas R Green, Guido Kroemer
Sep 24, 2004·The Journal of Cell Biology·Silke WissingFrank Madeo
Nov 5, 2004·The EMBO Journal·Nicola VahsenGuido Kroemer
Nov 24, 2004·Current Opinion in Microbiology·Frank MadeoKai-Uwe Fröhlich
Feb 12, 2009·Journal of Cell Science·Jane E LeadshamCampbell W Gourlay
Mar 3, 2009·Biochemical and Biophysical Research Communications·Frank MadeoGuido Kroemer
Nov 7, 2012·The International Journal of Biochemistry & Cell Biology·Serena BroggiSonia Colombo
Oct 4, 2013·Oxidative Medicine and Cellular Longevity·Loredana AmigoniSonia Colombo

❮ Previous
Next ❯

Citations

Feb 19, 2019·FEMS Yeast Research·Ramachandran GowsalyaVasanthi Nachiappan
Jul 16, 2020·Apoptosis : an International Journal on Programmed Cell Death·Maurizio D BaroniEnzo Martegani
Oct 13, 2018·International Journal of Molecular Sciences·Szymon KaczanowskiUrszula Zielenkiewicz
Aug 1, 2019·Antonie van Leeuwenhoek·Ramachandran GowsalyaVasanthi Nachiappan
Jul 13, 2021·Frontiers in Cell and Developmental Biology·Susana R ChavesManuela Côrte-Real
Mar 15, 2021·Current Genetics·Ramachandran GowsalyaVasanthi Nachiappan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.