Involvement of alanine racemase in germination of Bacillus cereus spores lacking an intact exosporium

Archives of Microbiology
Elena VenirMara Lucia Stecchini

Abstract

The L-alanine mediated germination of food isolated Bacillus cereus DSA 1 spores, which lacked an intact exosporium, increased in the presence of D-cycloserine (DCS), which is an alanine racemase (Alr) inhibitor, reflecting the activity of the Alr enzyme, capable of converting L-alanine to the germination inhibitor D-alanine. Proteomic analysis of the alkaline extracts of the spore proteins, which include exosporium and coat proteins, confirmed that Alr was present in the B. cereus DSA 1 spores and matched to that encoded by B. cereus ATCC 14579, whose spore germination was strongly affected by the block of conversion of L- to D-alanine. Unlike ATCC 14579 spores, L-alanine germination of B. cereus DSA 1 spores was not affected by the preincubation with DCS, suggesting a lack of restriction in the reactant accessibility.

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Citations

Sep 18, 2014·Journal of Mass Spectrometry : JMS·Vincenzo CunsoloSalvatore Foti
Sep 3, 2011·PloS One·Cassandra TerryPer A Bullough
Oct 1, 2015·Environmental Microbiology·Sara B Hernández, Felipe Cava
Oct 30, 2015·Microbiology and Molecular Biology Reviews : MMBR·George C Stewart

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