Involvement of alpha 1-2-fucosyltransferase I (FUT1) and surface-expressed Lewis(y) (CD174) in first endothelial cell-cell contacts during angiogenesis

Journal of Cellular Physiology
Thomas M MoehlerReinhard Schwartz-Albiez

Abstract

During the initiation of tumor associated angiogenesis endothelial cells migrate, adhere to extracellular matrix and form cell-cell contacts. Humoral factors of malignant cells conduct this process. We investigated whether cell surface expression of the carbohydrate blood group determinant Lewis(y) (CD174) and its precursor structure H2 (CD173) on endothelial cells is influenced by soluble factors of tumor cells. Using a bone marrow derived endothelial cell line we observed an enhanced expression of CD173/CD174 and transcription of FUT1, the key enzyme for their synthesis, after treatment with tumor necrosis factor-alpha (TNF-alpha) or conditioned supernatants of the leukemia cell line KG1a. CD173/CD174 are concentrated on pseudopodial extensions responsible for initial contacts between endothelial cells. Endothelial migration induced by TNF-alpha could be diminished by antibodies to CD174 as well as by siRNA induced downmodulation of FUT1 transcription. Endothelial FUT1-siRNA-transfectants displayed impaired in vitro angiogenesis when cultivated on extracellular matrix and in spheroid assays. In vivo upregulation of CD174 expression was observed immunocytologically in capillaries of tumor-infiltrated tissue. Together, our obse...Continue Reading

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Citations

Feb 18, 2010·Angiogenesis·Martina Willhauck-FleckensteinReinhard Schwartz-Albiez
Oct 16, 2012·Angiogenesis·Pei-Suen TsouMohammad A Amin
Jun 18, 2009·Journal of Molecular Evolution·Joana AbrantesJacques Le Pendu
Oct 4, 2011·Vaccine·Jamie Heimburg-MolinaroKate Rittenhouse-Olson
Jan 6, 2017·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Luca MaroniUlrich Beuers
Mar 15, 2019·Glycobiology·Kathya Gutiérrez-HuanteIván Martínez-Duncker
Feb 3, 2016·International Journal of Cancer. Journal International Du Cancer·Satish Kumar DevarapuReinhard Schwartz-Albiez
Mar 26, 2014·Annals of the Rheumatic Diseases·Mohammad A AminAlisa E Koch
Jan 23, 2020·Proceedings of the National Academy of Sciences of the United States of America·Loic Le GuennecMathieu Coureuil
Mar 1, 2010·Oncology Letters·L Muinelo-RomayA Fernández-Briera
Mar 19, 2021·Arteriosclerosis, Thrombosis, and Vascular Biology·Cheng-Hsiang KuoHua-Lin Wu

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