PMID: 9187535Feb 1, 1997Paper

Involvement of arachidonic acid and the lipoxygenase pathway in mediating luteinizing hormone-induced testosterone synthesis in rat Leydig cells

Endocrine Research
P G MeleE J Podestá

Abstract

Evidence has been introduced linking the lipoxygenase products and steroidogenesis in Leydig cells, thereby supporting that this pathway may be a common event in the hormonal control of steroid synthesis. On the other hand, it has also been reported that lipoxygenase products of arachidonic acid (AA) may not be involved in Leydig cells steroidogenesis. In this paper, we investigated the effects of PLA2 and lipoxygenase pathway inhibitors on steroidogenesis in rat testis Leydig cells. The effects of two structurally unrelated PLA2 inhibitors (4-bromophenacyl bromide (BPB) and quinacrine) were determined. BPB blocked the LH- and Bt2cAMP-stimulated testosterone production but had no effect on 22(4)-OH-cholesterol conversion to testosterone. Quinacrine caused a dose-dependent inhibition of LH- and Bt2cAMP-induced steroidogenesis. The effects of different lipoxygenase pathway inhibitors (nordihydroguaiaretic acid (NDGA), 5,8,11,14-eicosatetraynoic acid (ETYA), caffeic acid and esculetin) have also been determined. Both NDGA and ETYA inhibited LH- and Bt2cAMP-stimulated steroid synthesis in a dose-related manner. Furthermore caffeic acid and esculetin also blocked the LH-stimulated testosterone production. Moreover, exogenous AA indu...Continue Reading

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Citations

Oct 13, 2006·Antioxidants & Redox Signaling·Anna M BarronRalph N Martins
Jan 9, 2007·Molecular and Cellular Endocrinology·P MalobertiE J Podestá
Aug 28, 2004·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·K H JanbazA H Gilani
Feb 7, 2015·Molecular and Cellular Endocrinology·Melina DattiloFabiana Cornejo Maciel
Nov 20, 2012·Molecular and Cellular Endocrinology·Mariana CookeFabiana Cornejo Maciel
Jan 18, 2003·Endocrine Research·Rocío Castilla LozanoErnesto J Podestá
Jun 30, 2019·Animals : an Open Access Journal From MDPI·Nina ZhangZhongliang Jiang
Dec 22, 1999·The American Journal of Physiology·D ConteL di Luigi
Jul 14, 2019·Prostaglandins & Other Lipid Mediators·Isabel NeumanFabiana Cornejo Maciel
Jan 12, 2000·Molecular and Cellular Endocrinology·X Wang, D M Stocco

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