Involvement of Bruton's tyrosine kinase in FcepsilonRI-dependent mast cell degranulation and cytokine production

The Journal of Experimental Medicine
D HataToshiaki Kawakami

Abstract

We investigated the role of Bruton's tyrosine kinase (Btk) in FcepsilonRI-dependent activation of mouse mast cells, using xid and btk null mutant mice. Unlike B cell development, mast cell development is apparently normal in these btk mutant mice. However, mast cells derived from these mice exhibited significant abnormalities in FcepsilonRI-dependent function. xid mice primed with anti-dinitrophenyl monoclonal IgE antibody exhibited mildly diminished early-phase and severely blunted late-phase anaphylactic reactions in response to antigen challenge in vivo. Consistent with this finding, cultured mast cells derived from the bone marrow cells of xid or btk null mice exhibited mild impairments in degranulation, and more profound defects in the production of several cytokines, upon FcepsilonRI cross-linking. Moreover, the transcriptional activities of these cytokine genes were severely reduced in FcepsilonRI-stimulated btk mutant mast cells. The specificity of these effects of btk mutations was confirmed by the improvement in the ability of btk mutant mast cells to degranulate and to secrete cytokines after the retroviral transfer of wild-type btk cDNA, but not of vector or kinase-dead btk cDNA. Retroviral transfer of Emt (= Itk/Ts...Continue Reading

References

Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J E HutchcroftJ M Oliver
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M BenhamouR P Siraganian
Jan 1, 1991·Annual Review of Immunology·J V Ravetch, J P Kinet
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D CaputA Cerami
Mar 30, 1989·Nature·M Reth
Jan 1, 1986·International Archives of Allergy and Applied Immunology·N InagakiA Koda
Jan 1, 1986·International Archives of Allergy and Applied Immunology·N InagakiA Koda
Jan 1, 1994·European Journal of Immunology·J Hasbold, G G Klaus
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·L YaoT Kawakami
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·A AugustB Dupont
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·S J SaouafJ B Bolen
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·Y AokiS Pillai
Sep 1, 1995·Immunity·W N KhanL A Herzenberg
Sep 1, 1995·Immunity·J D KernerR M Perlmutter
Jan 1, 1995·Immunity·S C Jameson, M J Bevan
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·W S PearD Baltimore
Feb 1, 1995·Biological & Pharmaceutical Bulletin·H NagaiH Mori
Feb 1, 1995·Immunology Today·J C Cambier
Aug 1, 1994·The Journal of Experimental Medicine·A E GoldfeldA Rao
Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·G ChengD Baltimore

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Citations

Jan 4, 2006·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·S K LundyN W Lukacs
Dec 4, 2010·Immunologic Research·Xiao-Ping ZhongHongjie Pan
Feb 14, 2006·Nature Reviews. Immunology·Alasdair M Gilfillan, Christine Tkaczyk
Jul 30, 2011·The Journal of Biological Chemistry·Lawren C Wu
Sep 9, 1998·The Journal of Experimental Medicine·A B SatterthwaiteO N Witte
May 17, 2000·The Journal of Experimental Medicine·U D BajpaiH H Wortis
Sep 7, 2000·The Journal of Experimental Medicine·J KitauraT Kawakami
Nov 20, 2002·The Journal of Experimental Medicine·Ryohei F TsujiPhilip W Askenase
Mar 16, 2005·The Journal of Experimental Medicine·Junko SasakiYasunori Kanaho
Oct 11, 2007·The Journal of Pharmacology and Experimental Therapeutics·Bettina M JensenAlasdair M Gilfillan
Dec 22, 1999·Infection and Immunity·S M Singer, T E Nash
Feb 25, 2000·Molecular and Cellular Biology·J V FalvoA E Goldfeld
Jun 8, 1999·Annual Review of Immunology·J P Kinet
Mar 23, 2004·Annual Review of Immunology·Jonathan A Deane, David A Fruman
Mar 22, 2005·American Journal of Respiratory Cell and Molecular Biology·Johan ForssellJonas S Erjefält
Jan 24, 2013·Cell Communication and Signaling : CCS·Michael Huber
Jan 12, 2008·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Naoki Inagaki
Jan 28, 2014·PloS One·Joan Ní GabhannCaroline A Jefferies
Aug 9, 2007·Proceedings of the National Academy of Sciences of the United States of America·Oliver HantschelGiulio Superti-Furga
Mar 15, 2006·Proceedings of the National Academy of Sciences of the United States of America·Janet KalesnikoffStephen J Galli
Jun 15, 2000·Proceedings of the National Academy of Sciences of the United States of America·Y KawakamiT Kawakami
Apr 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·J V FalvoA E Goldfeld
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y KawakamiT Kawakami

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Datasets Mentioned

BETA
GM-CSF

Methods Mentioned

BETA
PCA
transfection
electrophoresis
PCR
ELISA
Assay
light microscopy
protein kinase C
transfectant
nuclear translocation

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Anaphylaxis

Anaphylaxis is a serious allergic reaction that is rapid in onset and may cause death.

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