Involvement of CaMK-IIδ and gelsolin in Cd(2+) -dependent cytoskeletal effects in mesangial cells

Journal of Cellular Physiology
Ying Liu, Douglas M Templeton

Abstract

Cadmium is a toxic metal with pleiotropic effects on cell death and survival. The mesangial cell is particularly responsive to Cd's effects on kinase signaling pathways and cytoskeletal dynamics. Here we show that CaMK-II is a participant in the cytoskeletal effects of Cd(2+) . A major mesangial cell isoform, CaMK-IIδ, was identified in pellets of DNase I pull-downs and cytosolic immunoprecipitates of G-actin. CaMK-IIδ was also present in Triton X-100-insoluble cytoskeletal preparations and translocated to the cytoskeleton in a concentration-dependent manner in Cd-treated cells. Translocation was suppressed by KN93, an inhibitor of CaMK-II phosphorylation. In vitro actin polymerization studies indicated that recombinant CaMK-IIδ sequestered actin monomer. Cytoskeletal preparations from Cd-treated cells decrease the rate of polymerization, but KN93 co-treatment prevents this effect. Over-expressed CaMK-IIδ also translocated to the cytoskeleton upon Cd exposure, and this was prevented by KN93. Conversely, siRNA silencing of CaMK-IIδ increases the effect of cytoskeletal extracts on actin polymerization, and abrogates the effect of Cd. The actin capping and severing protein, gelsolin, translocates to the cytoskeleton in the presenc...Continue Reading

References

Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M N WaxhamP T Kelly
Jan 1, 1996·Cell Motility and the Cytoskeleton·Z WangD M Templeton
Jul 1, 1996·Toxicology and Applied Pharmacology·Z Wang, D M Templeton
Jan 19, 1999·Annual Review of Cell and Developmental Biology·A Schmidt, M N Hall
Nov 24, 1999·The Journal of Biological Chemistry·H Q SunH L Yin
Feb 5, 2000·Cell Motility and the Cytoskeleton·N L Freeman, J Field
Jul 26, 2000·Nature·J R LarisonJ G Crock
Sep 6, 2001·The Journal of Biological Chemistry·N CaranR M Tombes
Mar 9, 2004·Molecular Biology of the Cell·Enni BertlingPekka Lappalainen
Nov 11, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Margarita D ApostolovaDouglas M Templeton
Jun 13, 2006·The Journal of Clinical Investigation·Johannes BacksEric N Olson
Feb 13, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Rachel J JonesHarold A Singer
Apr 4, 2007·Proceedings of the National Academy of Sciences of the United States of America·Ken-Ichi OkamotoYasunori Hayashi
Jul 7, 2007·American Journal of Physiology. Cell Physiology·Matthew W C ChanChristopher A McCulloch
Apr 4, 2008·American Journal of Physiology. Cell Physiology·Melissa Z MercureHarold A Singer
May 20, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Santhosh K ManiDhandapani Kuppuswamy
May 29, 2008·Journal of Cellular Physiology·Ying Liu, Douglas M Templeton
Jul 10, 2008·Cell Motility and the Cytoskeleton·Charles A EasleyRobert M Tombes
Oct 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Yu-Chih Lin, Lori Redmond
Feb 12, 2009·The Journal of Biological Chemistry·Hugo SanabriaM Neal Waxham
Feb 24, 2009·Toxicology and Applied Pharmacology·Weiqun XiaoDouglas M Templeton
Apr 4, 2009·Toxicology and Applied Pharmacology·Gunnar F Nordberg
Apr 18, 2009·Toxicology and Applied Pharmacology·Frank Thévenod
Apr 18, 2009·Toxicology and Applied Pharmacology·Pius Joseph
May 13, 2009·Toxicology and Applied Pharmacology·Bruce A Fowler
Mar 30, 2010·Chemico-biological Interactions·Douglas M Templeton, Ying Liu
Apr 16, 2010·Canadian Journal of Physiology and Pharmacology·Ying Liu, Douglas M Templeton
Jan 25, 2012·The American Journal of the Medical Sciences·Jian-Wu ZhaoYan Wang

❮ Previous
Next ❯

Citations

Jan 28, 2014·Chemico-biological Interactions·Grace ChoongDouglas M Templeton
Feb 2, 2013·Canadian Journal of Physiology and Pharmacology·Douglas M Templeton, Ying Liu
Apr 24, 2018·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jia-Qiao ZhuZong-Ping Liu
Mar 7, 2013·Journal of Cellular Biochemistry·Grace ChoongDouglas M Templeton

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.