Abstract
Fibronectin (FN) is the foremost proliferation-associated extracellular matrix component promoting cell adhesion, migration, and survival. We examined the effect of FN on cell proliferation and the related signaling pathways in mouse embryonic stem (ES) cells. FN increased integrin β1, Src, focal adhesion kinase (FAK), and caveolin-1 phosphorylation levels in a time-dependent manner. Phosphorylation of Src, FAK, and caveolin-1 was attenuated by integrin β1 neutralizing antibody. Integrin β1, Src, and FAK coimmunoprecipitated with caveolin-1 in the presence of FN. In addition, FN increased RhoA and Rho kinase activation, which were completely blocked by PP2, FAK small interfering RNA (siRNA), caveolin-1 siRNA, or the caveolar disruptor methyl-β-cyclodextrin (MβCD). FN also increased phosphorylation of Akt and ERK 1/2, which were significantly blocked by either FAK siRNA, caveolin-1 siRNA, MβCD, GGTI-286 (RhoA inhibitor), or Y-27632 (Rho kinase inhibitor). FN-induced increase of protooncogenes (c-fos, c-myc, and c-Jun) and cell-cycle regulatory proteins (cyclin D1/CDK4 and cyclin E/CDK2) expression levels were attenuated by FAK siRNA or caveolin-1 siRNA. Furthermore, inhibition of each pathway such as integrin β1, Src, FAK, caveo...Continue Reading
References
May 1, 1987·Developmental Biology·A G Smith, M L Hooper
Mar 1, 1993·Pharmaceutical Research·C M BrettK M Giacomini
Apr 16, 1998·The Journal of Biological Chemistry·T OkamotoM P Lisanti
Jul 2, 1998·Biochemical and Biophysical Research Communications·D GingrasR Béliveau
Sep 19, 1998·Cell·K K WaryF G Giancotti
Sep 10, 1999·The Journal of Biological Chemistry·A R AsthagiriD A Lauffenburger
Jul 11, 2000·Molecular Biology of the Cell·S K Kuwada, X Li
Sep 2, 2000·The Journal of Biological Chemistry·A V BakinC L Arteaga
Jan 3, 2001·The Journal of Cell Biology·E H DanenK M Yamada
Nov 1, 2001·Annual Review of Cell and Developmental Biology·I L WeissmanF Gage
Nov 1, 2001·Annual Review of Cell and Developmental Biology·A G Smith
Aug 8, 2002·Oncogene·Ludmila JirmanovaPierre Savatier
Feb 22, 2003·The Journal of Biological Chemistry·Charito BuensucesoSanford J Shattil
Mar 19, 2003·Oncogene·Séverine Cruet-HennequartShoukat Dedhar
Mar 26, 2003·Journal of Cellular Physiology·Antonella MuscellaSanto Marsigliante
Mar 26, 2003·Cell·Stephen C Harrison
Aug 15, 2003·The Journal of Biological Chemistry·Xiaowu LiDaniel M Ramos
Oct 22, 2003·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Nikhat D BoydNils O Petersen
Jul 3, 2004·Cancer Research·Reju KorahRobert Wieder
Sep 24, 2004·Physiological Reviews·Alex W CohenMichael P Lisanti
Feb 3, 2005·The Journal of Clinical Endocrinology and Metabolism·Maddalena IllarioMario Vitale
Jun 25, 2005·Biochemical and Biophysical Research Communications·Enming ZhangXiaoxiang Zheng
Oct 29, 2005·Nature Genetics·Geraldine Guasch, Elaine Fuchs
Nov 5, 2005·Journal of Neurochemistry·Jan Wang, Richard Milner
Jan 13, 2006·Oncogene·S WatanabeT Nakano
Jan 31, 2006·Cancer Science·Mitsuhiro MatsuoIkuo Saiki
Mar 7, 2006·Oncogene·S W Han, J Roman
Apr 29, 2006·Human Molecular Genetics·Lyle ArmstrongMajlinda Lako
Jun 27, 2006·Oncogene·M SalatinoP V Elizalde
Aug 3, 2006·The Journal of Biological Chemistry·Shouwei HanJesse Roman
Dec 25, 2007·The Journal of Biological Chemistry·Camilla L OxleyKate L Wegener
Apr 23, 2008·Growth Factors·Jung Sun HeoHo Jae Han
Apr 25, 2008·American Journal of Physiology. Renal Physiology·Fangfang PengJoan C Krepinsky
Jun 3, 2008·Trends in Cell Biology·Richard K Assoian, Eric A Klein
Jun 28, 2008·Endocrinology·Laura C MatthewsMelissa Westwood
Oct 17, 2008·Cancer Research·Bharat JoshiIvan R Nabi
Mar 7, 2009·Cellular and Molecular Life Sciences : CMLS·M Y LeeH J Han
Mar 26, 2009·Genome Biology·Laura M HeiserPaul T Spellman
Mar 27, 2009·Cellular and Molecular Life Sciences : CMLS·S P YunH J Han
Apr 28, 2009·Molecular BioSystems·Jeffrey D RitzenthalerJesse Roman
Apr 29, 2009·The Journal of Cell Biology·Patrick LajoieIvan R Nabi
May 28, 2009·Journal of Neuroscience Research·Jianguo HuXiao-Ming Xu
Jun 23, 2009·Stem Cells·Min Young LeeHo Jae Han
Nov 20, 2009·Cell Cycle·Jean-François JasminMichael P Lisanti
Citations
Jan 19, 2013·Cell Death & Disease·C MeyerS Dooley
Oct 14, 2010·The Journal of Biological Chemistry·Corina M BorzaAmbra Pozzi
Sep 29, 2011·The Journal of Biological Chemistry·Alessandro PrinettiSandro Sonnino
Sep 24, 2011·Journal of Oncology·Stephan Niland, Johannes A Eble
Oct 22, 2013·PloS One·Yaoyao ZhanWei Zou
Nov 19, 2013·PloS One·Noelia LosinoAlejandra Guberman
Dec 18, 2013·Pharmacology & Therapeutics·Jianliang Zhang, Steven N Hochwald
Aug 3, 2014·Carcinogenesis·Zhiyu WangJianping Chen
Jan 19, 2016·Journal of Molecular and Cellular Cardiology·Iñigo Valiente-AlandiBurns C Blaxall
Nov 22, 2012·Journal of Cellular Physiology·Liv Ingrid FlinderEllen Skarpen
Feb 24, 2011·Journal of Cellular Physiology·Han Na Suh, Ho Jae Han
Nov 5, 2013·Matrix Biology : Journal of the International Society for Matrix Biology·Paulina Moreno-Layseca, Charles H Streuli
Jun 25, 2014·Muscle & Nerve·Zachary A GrahamPhilip M Gallagher
Sep 2, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Zohreh Jaafari-AshkavandiZahra Azizi
Mar 5, 2013·Stem Cell Research·Han Na Suh, Ho Jae Han
Jun 12, 2012·Biochimica Et Biophysica Acta·Jae Hong ParkHo Jae Han
Oct 11, 2012·International Journal of Cardiology·Sung Hyun ChoiSang Hong Baek
Jun 12, 2012·Cell Cycle·Kiran Kumar VelpulaJasti S Rao
May 11, 2016·Biochimica Et Biophysica Acta·Ming-Han LiuYue Zhou
Nov 9, 2016·Scientific Reports·Yi-Jen HsuehDavid Hui-Kang Ma
May 5, 2017·PLoS Computational Biology·William R HolmesLeah Edelstein-Keshet
Nov 28, 2017·Current Stem Cell Reports·Loriana Vitillo, Susan J Kimber
Dec 5, 2012·The Journal of International Medical Research·W Y MaQ Sun
Jan 21, 2014·Advanced Healthcare Materials·Karina H NakayamaNgan F Huang
Apr 2, 2014·Journal of Neuroscience Research·Maosheng Xia, Yue Zhu
Jul 9, 2014·Molecular and Cellular Biology·Robert L KortumRobert E Lewis
Nov 22, 2018·Phytotherapy Research : PTR·Ronghua YangJulin Xie
Nov 1, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Damien LoubetBenoit Schneider
Aug 11, 2017·Scientific Reports·Juliette EzpeletaBenoit Schneider
Dec 10, 2019·Reproduction in Domestic Animals = Zuchthygiene·Penglei ShenFenghua Lu
Jan 1, 2020·Cell Biology International·Yoshikazu InohTadahide Furuno
Mar 21, 2020·International Journal of Cancer. Journal International Du Cancer·Martina RaudenskaMichal Masarik
Mar 25, 2016·American Journal of Physiology. Cell Physiology·Lili XuXinfeng Liu
May 17, 2018·Stem Cells International·Mi-Young MoonSang Won Suh
Mar 6, 2015·World Journal of Gastroenterology : WJG·Ya-Jun ChengLiang-Hua Wang
Jun 29, 2017·Proceedings of the National Academy of Sciences of the United States of America·JinSeok ParkAndre Levchenko
Jun 29, 2013·International Journal of Molecular Medicine·Xin ZengTingting Zhang
May 21, 2014·Oncology Reports·Wei MaPeng-Xia Zhang
May 26, 2016·Molecular Medicine Reports·Yongfu LuQiang He
Oct 18, 2019·Particle and Fibre Toxicology·Ching-Yi ChenChing-Hao Li
Jun 7, 2019·Translational Vision Science & Technology·Yi-Jen HsuehHung-Chi Chen
Dec 15, 2020·FEBS Letters·Natasha BuwaNagaraj Balasubramanian
Oct 23, 2020·The Journal of Membrane Biology·Natasha BuwaNagaraj Balasubramanian
Jul 24, 2021·Neoplasia : an International Journal for Oncology Research·Hiba GhuraInaam A Nakchbandi
Sep 10, 2021·Journal of Cancer Research and Clinical Oncology·Xingning LaiLixia Xiong
Sep 21, 2021·Physical Biology·Elisabeth G Rens, Leah Edelstein-Keshet
Aug 1, 2013·Stem Cell Research & Therapy·Natasha Baker, Rocky S Tuan