Involvement of CD16 in antibody-dependent enhancement of porcine reproductive and respiratory syndrome virus infection

The Journal of General Virology
Weihong GuYue Wang

Abstract

The immunological effect of porcine reproductive and respiratory syndrome disease virus (PRRSV) vaccines is thought to be influenced by a variety of host factors, in which antibody-dependent enhancement (ADE) of infection is one crucial factor. Here, we assessed the mechanism of ADE of PRRSV infection. First, we found that subneutralizing serum could induce ADE of PRRSV infection in porcine alveolar macrophages (PAMs). Quantitative PCR, Western blotting and flow cytometry revealed that CD16 is the most abundant Fcγ receptor (FcγR) expressed on the surface of PAMs; thus, the role of CD16 in ADE of PRRSV infection was examined in PAMs. By using functional blocking antibodies, we demonstrated that CD16 is involved in enhanced virus production in PRRSV-antibody immune complex-infected PAMs. Because PAMs co-express different FcγR isoforms, we evaluated the effects of CD16 in FcγR-non-bearing cells by transfection. Using these engineered cells, we found that CD16 could specifically bind to the PRRSV-antibody immune complex and subsequently mediate internalization of the virus, resulting in the generation of progeny virus. We also showed that efficient expression of CD16 required association of the FcR γ-chain. Together, our findings ...Continue Reading

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Citations

Jul 26, 2015·Veterinary Immunology and Immunopathology·Crystal L LovingFederico A Zuckermann
Mar 10, 2017·Journal of Virology·Xiaolei LuoYue Wang
Apr 16, 2019·Frontiers in Cellular and Infection Microbiology·Orlando A AcevedoAlexis M Kalergis
Feb 23, 2020·Medical Microbiology and Immunology·Xiaoen HeWei Zhao
Oct 29, 2020·Toxicology Reports·Ronald N KostoffAristidis Tsatsakis
Apr 18, 2017·Veterinary Immunology and Immunopathology·Michael C Rahe, Michael P Murtaugh

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