PMID: 22579005May 15, 2012Paper

Involvement of cholesterol membrane transporter Niemann-Pick C1-like 1 in the intestinal absorption of lutein

Journal of Pharmacy & Pharmaceutical Sciences : a Publication of the Canadian Society for Pharmaceutical Sciences, Société Canadienne Des Sciences Pharmaceutiques
Yuki SatoKen Iseki

Abstract

Lutein is a carotenoid mainly found in green leafy vegetables and is located in the macula lutea in the human eye. Since humans cannot synthesize lutein de novo, it must be digested as food. The physiological importance of an orally administered compound depends on its interaction with target tissues. It is therefore important to clarify the absorption mechanism in the intestine. Cholesterol membrane transporters Niemann-Pick C1 Like 1 (NPC1L1) and scavenger receptor class B type 1 (SR-B1) are involved in the intestinal absorption of highly lipophilic compounds including cholesterol. Ezetimibe, a selective inhibitor of intestinal NPC1L1, is the widespread lipid-lowering agent. It is important to investigate the possibility of food-drug interactions in order to prevent undesirable and harmful clinical consequences. The aim of this work was to determine whether NPC1L1, SR-B1 and other transporters are involved in absorption of lutein. Caco-2 cells were used for accumulation and permeability study of lutein. Lutein concentration was determined by an HPLC system. The cDNA of transporters was isolated from total RNA of Caco-2 cells, and the expression of these transporters was confirmed by RT-PCR (reverse transcription-polymerase ch...Continue Reading

Citations

Nov 8, 2018·Nutrients·Ana A O XavierAntonio Pérez-Gálvez
Mar 5, 2016·Biological & Pharmaceutical Bulletin·Yuto TakekawaMitsuru Sugawara
Dec 28, 2016·Biopharmaceutics & Drug Disposition·Shunsuke NashimotoMitsuru Sugawara
Mar 5, 2019·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Yuki Sato
Sep 14, 2019·Antioxidants·Ana Gabriela MurilloMaria Luz Fernandez
Oct 2, 2019·Lipids·Yuki ManabeTatsuya Sugawara
Oct 3, 2018·Current Opinion in Clinical Nutrition and Metabolic Care·Charles DesmarchelierPatrick Borel
Jun 17, 2021·Nutrition Research·Ishani Bhat, Bangera Sheshappa Mamatha
Jul 3, 2021·Foods·Ruth Conboy StephensonCatherine Stanton

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