PMID: 6409154Jul 14, 1983Paper

Involvement of chondroitin sulphate in preventing adhesive cellular interactions

Biochimica Et Biophysica Acta
G FibbiV Chiarugi

Abstract

Glycosaminoglycans isolated from native non-adhesive surfaces of both endothelial and mesothelial origin and from endothelial cells cultured in vitro were analyzed by electrophoresis and characterized by chemical and enzymatic breakdown. All the surfaces examined expose in vivo chondroitin 6-sulphate as the main glycosaminoglycan. Under in vitro culture, the exposure of chondroitin sulphate is reduced. Paper chromatography of hydrolysis products upon degradation by chondroitinase AC shows equal amounts of both 6- and 4-sulphated disaccharides. At the same time, the surfaces lose their non-adhesiveness to leukocytes. The addition of fibroblast growth factor to endothelial monolayers restores both non-adhesiveness to leukocytes and exposure of chondroitin sulphate. These results seem to indicate that the exposure of chondroitin sulphate is important in preventing cellular adhesion.

References

Oct 1, 1976·Cell Differentiation·G Augusti-Tocco, V P Chiarugi
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J FolkmanB R Zetter
Apr 1, 1979·Hoppe-Seyler's Zeitschrift für physiologische Chemie·F Jilek, H Hörmann
Oct 1, 1974·The Journal of Cell Biology·T Elsdale, J Bard
Jan 1, 1982·Advances in Cancer Research·K Alitalo, A Vaheri
Feb 1, 1980·Cell Differentiation·S VannucchiV P Chiarugi

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