PMID: 589447Nov 4, 1977

Involvement of complement in degeneration of sympathetic nerves after administration of antiserum to dopamine beta-hydroxylase

Brain Research
J B FurnessL B Geffen


The involvement of complement in the degeneration of noradrenergic nerves in the guinea-pig iris produced by administration of antibody to dopamine beta-hydroxylase (DBH) in vivo was investigated histochemically. When 500 microliter of antiserum to DBH was injected systemically, no evidence of degeneration was observed in the iris although the noradrenergic supply of the myenteric plexus of the ileum degenerated within 2 days. However, injection of 20 microliter of complement (C) into the anterior chamber of one eye within 2 days of the systemic administration of anti-DBH produced a degeneration of 50--90% of noradrenergic terminals in the iris, the nerves of the iris of the contralateral uninjected eye being unaffected. Electron microscopy confirmed the presence of degenerating nerve terminals in the iris. The extent of degeneration produced by addition of C decreased when C was injected at increasing intervals after the antiserum. Intraocular injection of 5 microliter of anti-DBH together with 20 microliter of C caused a substantial degeneration of noradrenergic nerves in the iris. In contrast, intraocular injection of the Fab'2 fragment of anti-DBH (which did not bind C, but still bound DBH in vitro and in vivo) failed to ca...Continue Reading

Related Concepts

Adrenergic Fibers
Hemolytic Complement
Cavia porcellus
Immune Sera
Iris (Eye)
Nerve Degeneration

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