Involvement of cyclin K posttranscriptional regulation in the formation of Artemia diapause cysts.

PloS One
Yang ZhaoWei-Jun Yang

Abstract

Artemia eggs tend to develop ovoviviparously to yield nauplius larvae in good rearing conditions; while under adverse situations, they tend to develop oviparously and encysted diapause embryos are formed instead. However, the intrinsic mechanisms regulating this process are not well understood. This study has characterized the function of cyclin K, a regulatory subunit of the positive transcription elongation factor b (P-TEFb) in the two different developmental pathways of Artemia. In the diapause-destined embryo, Western blots showed that the cyclin K protein was down-regulated as the embryo entered dormancy and reverted to relatively high levels of expression once development resumed, consistent with the fluctuations in phosphorylation of position 2 serines (Ser2) in the C-terminal domain (CTD) of the largest subunit (Rpb1) of RNA polymerase II (RNAP II). Interestingly, the cyclin K transcript levels remained constant during this process. In vitro translation data indicated that the template activity of cyclin K mRNA stored in the postdiapause cyst was repressed. In addition, in vivo knockdown of cyclin K in developing embryos by RNA interference eliminated phosphorylation of the CTD Ser2 of RNAP II and induced apoptosis by i...Continue Reading

References

Apr 16, 1998·Genes & Development·J PengD H Price
Mar 25, 2000·Molecular and Cellular Biology·D H Price
Mar 30, 2001·The Journal of Biological Chemistry·Y RamanathanT Pe'ery
Mar 9, 2002·The Journal of Biological Chemistry·Xin LinB Matija Peterlin
Apr 5, 2003·Annual Review of Biochemistry·Ali ShilatifardJoan Weliky Conaway
Sep 27, 2003·European Journal of Biochemistry·Benoît Palancade, Olivier Bensaude
Mar 30, 2004·The Journal of Biological Chemistry·Janice C JonesArno L Greenleaf
Oct 19, 2004·Genes & Development·Robert J SimsDanny Reinberg
Jan 24, 2006·Biochemical and Biophysical Research Communications·Supansa YodmuangSakol Panyim
Aug 4, 2006·Molecular Cell·B Matija Peterlin, David H Price
Mar 14, 2007·Journal of Cellular Physiology·Eleonora LeucciAntonio Giordano
Mar 4, 2009·Journal of Veterinary Science·Hyun Sun ChoMyung Haing Cho
Feb 22, 2011·Apoptosis : an International Journal on Programmed Cell Death·Ning GaoXianglin Shi

❮ Previous
Next ❯

Citations

Jan 4, 2015·Fish & Shellfish Immunology·Chao-qun ZhengHai-peng Liu
Sep 26, 2013·The Biochemical Journal·Fan YangWei-Jun Yang
Jan 9, 2019·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Hajer Salem MalitanThomas H MacRae
Apr 8, 2016·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Steven C HandRichard Roy

❮ Previous
Next ❯

Datasets Mentioned

BETA
JQ085432
U10331
AF427598

Methods Mentioned

BETA
Affinity Chromatography
electrophoresis
co-immunoprecipitation

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.