Involvement of cyclooxygenase-2 in rat models of conjunctivitis

Current Eye Research
Takayuki OkaMitsuyoshi Azuma

Abstract

Using two animal models to determine which isoform of cyclooxygenase (COX), constitutive COX-1 or inducible COX-2, is involved in the progression of anterior ocular inflammation. Lambda-carrageenan (500 mg/eye) or bacterial lipopolysaccharide (LPS; 3 mg/eye) was injected into rat conjunctiva to induce conjunctivitis. Vascular permeability in inflamed conjunctiva was measured by uptake of systemic Evans blue. Changes in mRNA for COX-1 and COX-2 in conjunctiva were detected by RT-PCR. Changes in COX-2 protein were detected by immunoblotting after immunoprecipitation. To assess involvement of COX-2 in carrageenan and LPS-induced conjunctivitis, NS-398 (a selective COX-2 inhibitor) or indomethacin (non-selective COX inhibitor) was topically administrated at 15 and 30 minutes before inflammatory stimulator-injection. In the carrageenan-injected model, the dye content of conjunctiva (12.4 +/- 2.8 mg/eye) was significantly increased 4 hours after injection compared to saline-injected control rats (3.7 +/-1.1 mg/eye). mRNA for COX-2 was significantly increased by 2 hours and gradually increased until 24 hours; COX-1 mRNA did not show major changes until 24 hours after injection. COX-2 protein was markedly elevated 4 hours after injecti...Continue Reading

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Citations

Mar 22, 2008·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Zaher A Radi, James A Render
Feb 3, 2007·American Journal of Veterinary Research·Carey L McInnisJames R Turk
Aug 28, 2007·Journal of Cataract and Refractive Surgery·Tom WaltersRobert Lehmann
Dec 17, 2008·Veterinary Ophthalmology·Kerry M SmithJane Sansom
Sep 30, 2014·Journal of Cataract and Refractive Surgery·Ella G Faktorovich, Karishma Melwani
Jul 19, 2011·Ophthalmology·Bonnie A HendersonUNKNOWN Bromfenac Ophthalmic Solution (Bromday) Once Daily Study Group
Jun 13, 2015·Asia-Pacific Journal of Ophthalmology·Hiroshi FujishimaDogru Murat
Feb 11, 2020·Korean Journal of Ophthalmology : KJO·Seok Hyeon SongYoung Hoon Lee
Dec 1, 2007·European Journal of Ophthalmology·U AdiguzelL Cinel

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