PMID: 14684755Dec 20, 2003Paper

Involvement of CYP 2C9 in mediating the proinflammatory effects of linoleic acid in vascular endothelial cells

Journal of the American College of Nutrition
Saraswathi ViswanathanBernhard Hennig


Polyunsaturated fatty acids such as linoleic acid are well known dietary lipids that may be atherogenic by activating vascular endothelial cells. In the liver, fatty acids can be metabolized by cytochrome P450 (CYP) enzymes, but little is known about the role of these enzymes in the vascular endothelium. CYP 2C9 is involved in linoleic acid epoxygenation, and the major product of this reaction is leukotoxin (LTX). We investigated the role of CYP-mediated mechanisms of linoleic acid metabolism in endothelial cell activation by examining the effects of linoleic acid or its oxidized metabolites such as LTX and leukotoxin diol (LTD). The effect of linoleic acid on CYP 2C9 gene expression was studied by RT-PCR. Oxidative stress was monitored by measuring DCF fluorescence and intracellular glutathione levels, and electrophoretic mobility shift assay was carried out to study the activation of oxidative stress sensitive transcription factors. Analysis of oxidized lipids was carried out by liquid chromatography/mass spectrometry. Linoleic acid treatment for six hours increased the expression of CYP 2C9 in endothelial cells. Linoleic acid-mediated increase in oxidative stress and activation of AP-1 were blocked by sulfaphenazole, a speci...Continue Reading


Sep 1, 1984·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·Bernhard HennigArthur A Spector
Jan 1, 1980·Annual Review of Biochemistry·R E White, M J Coon
Oct 26, 1994·Molecular and Cellular Biochemistry·K KosakaT Ozawa
Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·J Y ParkB N Ames
Jun 17, 1998·Free Radical Biology & Medicine·S Puntarulo, A I Cederbaum
Nov 21, 1998·Free Radical Biology & Medicine·L BenovI Fridovich
Mar 5, 1999·Drug Metabolism Reviews·P E ScarboroughDarryl C Zeldin
Sep 25, 1999·Journal of Cellular Physiology·U HerbstBernhard Hennig
Mar 24, 2000·Archives of Biochemistry and Biophysics·A J Draper, Bruce D Hammock
Oct 24, 2000·Toxicology and Applied Pharmacology·J H MoranD F Grant
Mar 14, 2001·Trends in Biochemical Sciences·D R Davydov
Jun 27, 2001·Circulation·L A Moyé
Oct 27, 2001·Circulation Research·Ingrid Fleming
Jan 5, 2002·The American Journal of Clinical Nutrition·Michal ToborekBernhard Hennig
May 29, 2002·The Journal of Nutritional Biochemistry·Yong Woo LeeMichal Toborek
Jul 20, 2002·Methods in Enzymology·Michal ToborekBernhard Hennig
Nov 5, 2002·The American Journal of Pathology·Yao SunKarl T Weber
Nov 12, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Thomas MünzelDavid G Harrison
Nov 15, 2002·Methods : a Companion to Methods in Enzymology·Sang-Hyun Park, Ronald T Raines
Dec 3, 2002·The American Journal of Cardiology·Dominik Behrendt, Peter Ganz
Dec 14, 2002·The International Journal of Biochemistry & Cell Biology·Sung-Hee ParkYoung-Hee Kang


Apr 12, 2012·Cellular and Molecular Life Sciences : CMLS·Scott GleimJohn Hwa
Mar 19, 2013·Cardiovascular Toxicology·Anna Maria PosadinoGianfranco Pintus
Apr 17, 2007·Journal of Biosciences·David Alan Thompson, Bruce D Hammock
Jun 24, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Matthew L EdinDarryl C Zeldin
Oct 2, 2012·Annual Review of Pharmacology and Toxicology·Christophe Morisseau, Bruce D Hammock
May 20, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Limin WangJohn C Rutledge
Jul 11, 2009·Cellular and Molecular Life Sciences : CMLS·Doriane RichardFrancesco Visioli
Jul 9, 2013·Metabolomics : Official Journal of the Metabolomic Society·Angela M ZivkovicBruce D Hammock
Jun 10, 2009·Expert Opinion on Drug Metabolism & Toxicology·Rüdiger Kaspera, Rheem A Totah
Dec 23, 2015·Biochemical and Biophysical Research Communications·Viswanathan SaraswathiAlexander V Kabanov
Sep 28, 2010·Biochimica Et Biophysica Acta·Anne Konkel, Wolf-Hagen Schunck
Nov 8, 2008·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Kevin L Fritsche
Mar 8, 2005·Progress in Lipid Research·John W NewmanBruce D Hammock
Apr 12, 2005·Environmental Research·Iván N Pérez-MaldonadoLeticia Yáñez
Aug 1, 2013·Biochimie·Benjamin ChoquePhilippe Legrand
Feb 7, 2015·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Anna Maria PosadinoGianfranco Pintus
May 27, 2016·Proceedings of the National Academy of Sciences of the United States of America·Derek W GilroyDavid Bishop-Bailey
Dec 7, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ganna M ShayakhmetovaValentina M Kovalenko
Mar 3, 2004·Journal of Lipid Research·Viswanathan SaraswathiBernhard Hennig
Jun 8, 2018·Acta Pharmacologica Sinica·Mohammed A Nayeem
Sep 18, 2007·Cell Biochemistry and Function·B ErcanL Tamer
Feb 7, 2019·Cardiovascular Toxicology·Marwin BannehrAlexander Wutzler
Apr 25, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·David C NiemanKirk L Pappan
Aug 14, 2019·The British Journal of Nutrition·Afroza FerdouseHarold M Aukema
Oct 9, 2019·The Journal of International Medical Research·Yue ChangHai Li
Oct 18, 2007·Current Opinion in Endocrinology, Diabetes, and Obesity·Sheila M Innis
Dec 6, 2017·PloS One·G Andres ContrerasLorraine M Sordillo
Oct 4, 2017·Scientific Reports·Poonamjot DeolFrances M Sladek
Jul 24, 2019·Frontiers in Pharmacology·Ingrid Fleming
Oct 10, 2008·The American Journal of Clinical Nutrition·Ahmad Esmaillzadeh, Leila Azadbakht
Aug 13, 2017·American Journal of Physiology. Heart and Circulatory Physiology·Stephanie P B CaligiuriHarold M Aukema
Aug 16, 2017·Research in Veterinary Science·Vengai Mavangira, Lorraine M Sordillo
May 19, 2009·Chemical Research in Toxicology·Céline NarjozIsabelle De Waziers

Related Concepts

LTX, Actinobacillus actinomycetemcomitans
9,10-dihydroxy-12-octadecenoic acid
CYP2C9 protein, human
Xenobiotic Monooxygenases
Dose-Response Relationship, Immunologic
Endothelium, Vascular
Reduced Glutathione
Immunosuppressive Agents
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