Involvement of cysteine-rich protein 61 in the epidermal growth factor-induced migration of human anaplastic thyroid cancer cells

Molecular Carcinogenesis
Li-Han ChinYu-Chih Liang

Abstract

Anaplastic thyroid cancer (ATC) is among the most aggressive types of malignant cancer. Epidermal growth factor (EGF) plays a crucial role in the pathogenesis of ATC, and patients with thyroid carcinoma typically exhibit increased cysteine-rich protein 61 (Cyr61). In this study, we found that EGF treatment induced cell migration, stress fiber formation, Cyr61 mRNA and protein expressions, and Cyr61 protein secretion in ATC cells. The recombinant Cyr61 protein significantly induced cell migration; however, inhibition of Cyr61 activity by a Cyr61-specific antibody abrogated EGF-induced cell migration. EGF treatment also affected epithelial-to-mesenchymal transition (EMT)-related marker protein expression, as evidenced by an increase in vimentin and a decrease in E-cadherin expression. Inhibition of Cyr61 expression by Cyr61 siRNA decreased cell migration and reversed the EMT-related marker protein expression. EGF treatment increased the phosphorylation of the extracellular signal-regulated kinase (ERK) and cAMP response element-binding protein (CREB), and finally activated Cyr61 promoter plasmid activity. Our results suggest that Cyr61 is induced by EGF through the ERK/CREB signal pathway and that it plays a crucial role in the m...Continue Reading

References

Jun 1, 1992·British Journal of Cancer·D R HaugenJ R Lillehaug
Nov 22, 1991·Science·S A Aaronson
Apr 20, 1990·Cell·A Ullrich, J Schlessinger
Feb 1, 1995·Thyroid : Official Journal of the American Thyroid Association·B F van der LaanS L Asa
Dec 11, 1999·Nucleic Acids Research·E WingenderF Schacherer
Apr 6, 2001·Thyroid : Official Journal of the American Thyroid Association·U MartiU Bürgi
Apr 12, 2001·The Journal of Biological Chemistry·D XieH P Koeffler
Feb 13, 2002·Oncogene·Miaw-Sheue TsaiRuth Lupu
Aug 6, 2003·European Journal of Biochemistry·Ji-Soo HanBrahim Chaqour
Oct 9, 2004·The Journal of Biological Chemistry·Wenwen ChienH Phillip Koeffler
Dec 30, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Bradley A SchiffJeffrey N Myers
Jan 22, 2005·Annals of the New York Academy of Sciences·C EnsingerK W Schmid
Mar 17, 2005·Frontiers in Bioscience : a Journal and Virtual Library·Anne-Marie BleauBernard Perbal
Aug 24, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ming-Tsai LinMin-Liang Kuo
Mar 14, 2008·The New England Journal of Medicine·Fortunato Ciardiello, Giampaolo Tortora
Jul 28, 2009·The Journal of Biological Chemistry·Andrey S DobroffMenashe Bar-Eli
Jan 26, 2010·International Journal of Surgery·Rajeev ParameswaranGregory Paul Sadler
Jan 21, 2011·International Journal of Molecular Medicine·Jian-Jun XieEn-Min Li
Feb 12, 2011·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Olivia FromiguePierre J Marie
Aug 2, 2011·Cellular and Molecular Life Sciences : CMLS·Lester F Lau
Nov 4, 2011·Cancer Immunology, Immunotherapy : CII·Jinpiao LinNingli Li
Feb 22, 2012·Asian Journal of Andrology·Naoki TeradaRobert H Getzenberg
Mar 10, 2012·Reproduction, Fertility, and Development·Rebecca KleinIsabella Gashaw

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Citations

Feb 7, 2018·Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre·Canan DemirNeslihan B Tutuncu
Dec 14, 2017·International Journal of Molecular Sciences·Mei-Chieh ChenSung-Po Hsu
May 13, 2018·International Journal of Molecular Sciences·Fat-Moon SukYu-Chih Liang

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