Involvement of distinct G-proteins in the action of vasopressin on rat glomerulosa cells

Endocrinology
G GuillonN Gallo-Payet

Abstract

We have previously shown that vasopressin (VP) induces breakdown of membrane phosphoinositides in adrenal glomerulosa cells. In the present study we demonstrate that the accumulation of inositol phosphates (IP) measured in the presence of arginine vasopressin (AVP) is reduced if the cells are incubated in a calcium-free medium. This effect cannot be accounted for by modification of VP binding, reduction of inositol lipid labeling, or stimulation of inositol, 1,4,5,-triphosphate 5-monophosphatase. It mainly affects phospholipase-C activity, since this enzyme is highly sensitive to calcium. Ionomycine and nifedipine, which, respectively, increase and decrease the intracellular calcium concentration, also, respectively, stimulate and inhibit IP accumulation. In membranes prepared from pertussis toxin (IAP)-treated cells, AVP stimulates inositol monophosphate accumulation to the same extent as in membranes derived from untreated cells. However, in intact cells, IAP decreases the inositol monophosphate accumulation. This decrease probably involves calcium influx, since we show that AVP stimulates a unidirectional calcium influx, which is completely blocked by IAP treatment. In rat adrenal glomerulosa cells, the AVP-stimulated secret...Continue Reading

Citations

Mar 19, 1991·Biochimica Et Biophysica Acta·E MeldrumA Carozzi
Jan 1, 1992·Cellular Signalling·G GuillonA L Savage
Feb 3, 2009·Clinics in Chest Medicine·Virginie MaximeDjillali Annane
Aug 1, 1995·Cell Calcium·K MalyH H Grunicke
Mar 27, 2004·Physiological Reviews·András Spät, László Hunyady
Aug 19, 2004·American Journal of Physiology. Endocrinology and Metabolism·Judith A EnyeartJohn J Enyeart
May 7, 1998·Endocrine Reviews·M Ehrhart-BornsteinG P Vinson
Jul 1, 1993·The American Journal of Physiology·A TetiS Adamo

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