Involvement of Ech hydrogenase in energy conservation of Methanosarcina mazei

The FEBS Journal
Cornelia WelteUwe Deppenmeier

Abstract

Methanosarcina mazei belongs to the group of aceticlastic methanogens and converts acetate into the potent greenhouse gases CO(2) and CH(4). The aceticlastic respiratory chain involved in methane formation comprises the three transmembrane proteins Ech hydrogenase, F(420) nonreducing hydrogenase and heterodisulfide reductase. It has been shown that the latter two contribute to the proton motive force. The data presented here clearly demonstrate that Ech hydrogenase is also involved in energy conservation. ATP synthesis was observed in a cytoplasm-free vesicular system of Ms. mazei that was dependent on the oxidation of reduced ferredoxin and the formation of molecular hydrogen (as catalysed by Ech hydrogenase). Such an ATP formation was not observed in a Deltaech mutant strain. The protonophore 3,5-di-tert-butyl-4-hydroxybenzylidene-malononitrile (SF6847) led to complete inhibition of ATP formation in the Ms. mazei wild-type without inhibiting hydrogen production by Ech hydrogenase, whereas the sodium ion ionophore ETH157 did not affect ATP formation in this system. Thus, we conclude that Ech hydrogenase acts as primary proton pump in a ferredoxin-dependent electron transport system.

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Citations

Oct 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Eva Biegel, Volker Müller
Jul 20, 2012·The Journal of Biological Chemistry·Kai Schuchmann, Volker Müller
Jul 10, 2013·Journal of Bacteriology·Ricardo Jasso-ChávezJames G Ferry
Jul 26, 2011·BMC Microbiology·Mingyu WangJames G Ferry
Jul 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jae Kyu LimVolker Müller
Nov 11, 2014·Nature Reviews. Microbiology·Kai Schuchmann, Volker Müller
Dec 18, 2013·Biochimica Et Biophysica Acta·Cornelia Welte, Uwe Deppenmeier
Jan 30, 2013·Biochemical Society Transactions·Katharina Schlegel, Volker Müller
Sep 18, 2012·Biochimica Et Biophysica Acta·Barbara Schoepp-CothenetWolfgang Nitschke
Oct 15, 2013·FEMS Microbiology Reviews·Florian Mayer, Volker Müller
Mar 6, 2012·Biochimica Et Biophysica Acta·Rouslan G Efremov, Leonid A Sazanov
Sep 25, 2012·Biochimica Et Biophysica Acta·Bruno C MarreirosManuela M Pereira
Mar 23, 2017·Microbes and Environments·Shawn E McGlynn
Aug 23, 2017·BMC Genomics·Sean P GilmoreMichelle A O'Malley
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Jun 21, 2018·Annual Review of Microbiology·Volker MüllerMirko Basen
Aug 27, 2013·Journal of Bacteriology·Fabio O Morais-SilvaClaudina Rodrigues-Pousada
Jan 20, 2019·Applied and Environmental Microbiology·Sarah Piché-Choquette, Philippe Constant
Aug 20, 2019·Molecular Microbiology·Alexander J FinneyFrank Sargent
Sep 20, 2019·Microbiology and Molecular Biology Reviews : MMBR·Thomas D Mand, William W Metcalf
Oct 28, 2019·Applied and Environmental Microbiology·Nikola PalevichGraeme T Attwood
Jun 17, 2020·Applied Microbiology and Biotechnology·Julia M KurthCornelia U Welte
Mar 10, 2019·Proceedings of the National Academy of Sciences of the United States of America·Marie Charlotte Schoelmerich, Volker Müller
Dec 28, 2019·Proceedings of the National Academy of Sciences of the United States of America·Marie Charlotte SchoelmerichVolker Müller
Oct 21, 2019·Cellular and Molecular Life Sciences : CMLS·Marie Charlotte Schoelmerich, Volker Müller
Jan 30, 2019·Nature Communications·Paula B Matheus CarnevaliJillian F Banfield
Jan 6, 2021·Chemical Reviews·Filipa CalistoManuela M Pereira
Jan 12, 2021·Frontiers in Bioengineering and Biotechnology·Alexander Katsyv, Volker Müller
Oct 2, 2020·Biochimica Et Biophysica Acta. Bioenergetics·Stefanie BergerCornelia U Welte
Sep 5, 2021·Extremophiles : Life Under Extreme Conditions·Florian P Rosenbaum, Volker Müller

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