PMID: 9557669Apr 29, 1998Paper

Involvement of endoplasmic reticulum chaperones in the folding of hepatitis C virus glycoproteins

Journal of Virology
A ChoukhiJean Dubuisson

Abstract

The hepatitis C virus (HCV) genome encodes two envelope glycoproteins (E1 and E2) which interact noncovalently to form a heterodimer (E1-E2). During the folding and assembly of HCV glycoproteins, a large portion of these proteins are trapped in aggregates, reducing the efficiency of native E1-E2 complex assembly. To better understand this phenomenon and to try to increase the efficiency of HCV glycoprotein folding, endoplasmic reticulum chaperones potentially interacting with these proteins were studied. Calnexin, calreticulin, and BiP were shown to interact with E1 and E2, whereas no interaction was detected between GRP94 and HCV glycoproteins. The association of HCV glycoproteins with calnexin and calreticulin was faster than with BiP, and the kinetics of interaction with calnexin and calreticulin were very similar. However, calreticulin and BiP interacted preferentially with aggregates whereas calnexin preferentially associated with monomeric forms of HCV glycoproteins or noncovalent complexes. Tunicamycin treatment inhibited the binding of HCV glycoproteins to calnexin and calreticulin, indicating the importance of N-linked oligosaccharides for these interactions. The effect of the co-overexpression of each chaperone on the...Continue Reading

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Citations

Feb 2, 2002·Applied and Environmental Microbiology·Ana ConesaPeter J Punt
Oct 17, 2001·The Journal of General Virology·A Op De BeeckJ Dubuisson
Dec 28, 2002·Journal of Virology·Laurence CocquerelShoshana Levy
Jan 31, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·François PeninJean-Michel Pawlotsky
Jul 12, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shiu-Wan Chan, Philip Anthony Egan
Aug 13, 2008·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·B F F ChumpitaziC Drouet
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Jan 27, 2012·Journal of Virology·Kathleen McCaffreyHeidi E Drummer
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Jun 7, 2014·Frontiers in Microbiology·Shiu-Wan Chan
Aug 1, 2015·World Journal of Gastroenterology : WJG·Daniela Ploen, Eberhard Hildt
Jan 20, 2016·World Journal of Hepatology·Ronik Khachatoorian, Samuel W French
Mar 25, 2016·PloS One·Kunlakanya JitoboamDuncan R Smith
Jul 8, 2016·The Journal of General Virology·Richard A UrbanowiczAlexander W Tarr
Apr 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Davor PavlovićNicole Zitzmann
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Nov 24, 1999·The Journal of General Virology·A ChoukhiJ Dubuisson
Mar 1, 2003·The Journal of Biological Chemistry·Pierre-Yves LozachRalf Altmeyer

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