Involvement of EP1 and EP2 receptors in the regulation of the Na,K-ATPase by prostaglandins in MDCK cells

Prostaglandins & Other Lipid Mediators
Keikantse Matlhagela, Mary Taub

Abstract

Prostaglandins are key regulators of ion transport in the kidney. In MDCK cells, which model distal tubule cells, the transcription of the Na,K-ATPase beta1 subunit is regulated by PGE1 and PGE2. To identify the EP receptors that mediate transcriptional regulation, transient transfection studies are conducted using the human beta1promoter/luciferase construct, pHbeta1-1141 Luc. The involvement of EP1 and EP2 receptors is indicated by studies with the EP1 selective agonist 17-phenyl trinor PGE2, and the EP2 selective agonist butaprost (which stimulate), as well as by studies with the antagonists SC-51089 (EP1 specific) and AH 6809 (EP1 and EP2 specific). Consistent with the involvement of Gs coupled EP2 receptors, is that the PGE1 stimulation is inhibited by the PKAI expression vector (encoding the protein kinase A (PKA) inhibitory protein), as well as by the myristolated PKA inhibitory peptide PKI. In addition to this evidence (for the involvement of EP2 receptors), evidence for the involvement of EP1 receptors in the PGE1 mediated stimulation of Na,K-ATPase beta subunit gene transcription includes the stimulatory effect of 17-phenyl trinor PGE2, as well as the inhibitory effects of SC-51089. Also consistent with the involvemen...Continue Reading

References

Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J E Lever
Aug 1, 1979·Journal of Cellular Physiology·J D ValentichJ Leighton
Jan 1, 1979·Journal of Supramolecular Structure·M Taub, G H Sato
Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M TaubG Sato
Jun 1, 1978·The Journal of Cell Biology·M CereijidoD D Sabatini
Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·T C LeeR J Schwartz
Mar 1, 1991·Kidney International·J V Bonventre, R Nemenoff
Jun 30, 1989·Biochemical and Biophysical Research Communications·D W CoyneA R Morrison
Jan 1, 1980·The Journal of Membrane Biology·M CereijidoA Martínez-Palomo
Nov 9, 1995·Biochemical Pharmacology·D F WoodwardJ W Regan
Oct 29, 1993·Journal of Medicinal Chemistry·E A HallinanM Reichman
Dec 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·H R HerschmanS Reddy
Sep 12, 1998·The Journal of Biological Chemistry·D JeanM Bar-Eli
Oct 6, 1998·European Journal of Pharmacology·C R JanC J Tseng
Jun 8, 1999·The Journal of Clinical Investigation·S L TilleyB H Koller
Jul 15, 2000·American Journal of Physiology. Renal Physiology·M D Breyer, R M Breyer
Feb 22, 2001·Annual Review of Physiology·M D Breyer, R M Breyer
Jun 13, 2001·American Journal of Physiology. Cell Physiology·R T WorrellD C Eaton
Jul 1, 1958·Proceedings of the Society for Experimental Biology and Medicine·S H MADIN, N B DARBY
Oct 29, 2003·Prostaglandins, Leukotrienes, and Essential Fatty Acids·M Wegmann, R M Nüsing
Nov 11, 2003·Life Sciences·John W Regan
Feb 16, 2005·JPEN. Journal of Parenteral and Enteral Nutrition·Vishal BansalJuan B Ochoa

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Citations

Sep 18, 2013·Molecular Human Reproduction·Shubhendu Sen Roy, Polani B Seshagiri
Jun 8, 2013·American Journal of Respiratory Cell and Molecular Biology·Gregory E ConnerMatthias Salathe
Jan 24, 2012·Biochemical and Biophysical Research Communications·Rania NasrallahRichard L Hébert
Feb 16, 2010·Biochemical and Biophysical Research Communications·Mary TaubFacundo Cutuli
May 15, 2007·Prostaglandins & Other Lipid Mediators·Sawsan Ibrahim KreydiyyehAri Kassardjian
Jul 19, 2012·The FEBS Journal·Elizabeth Anne McRobertLeon A Bach
May 17, 2006·Biochemical and Biophysical Research Communications·Maryanne BorsickMary Taub
May 10, 2016·Biochemical and Biophysical Research Communications·Sarah E FiedlerSonemany Salinthone
Jul 3, 2009·American Journal of Physiology. Cell Physiology·D Flores-BenitezR G Contreras
Sep 1, 2007·Clinical Science·Rania NasrallahRichard L Hébert
Nov 14, 2008·American Journal of Physiology. Cell Physiology·Daniela SteinertWolfgang Neuhofer
Jun 3, 2021·International Journal of Molecular Sciences·Alejandro Ogazon Del ToroArturo Ponce

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