Involvement of exo5 in production of surface polysaccharides in Rhizobium leguminosarum and its role in nodulation of Vicia sativa subsp. nigra

Journal of Bacteriology
Marc C LausJ W Kijne

Abstract

Analysis of two exopolysaccharide-deficient mutants of Rhizobium leguminosarum, RBL5808 and RBL5812, revealed independent Tn5 transposon integrations in a single gene, designated exo5. As judged from structural and functional homology, this gene encodes a UDP-glucose dehydrogenase responsible for the oxidation of UDP-glucose to UDP-glucuronic acid. A mutation in exo5 affects all glucuronic acid-containing polysaccharides and, consequently, all galacturonic acid-containing polysaccharides. Exo5-deficient rhizobia do not produce extracellular polysaccharide (EPS) or capsular polysaccharide (CPS), both of which contain glucuronic acid. Carbohydrate composition analysis and nuclear magnetic resonance studies demonstrated that EPS and CPS from the parent strain have very similar structures. Lipopolysaccharide (LPS) molecules produced by the mutant strains are deficient in galacturonic acid, which is normally present in the core and lipid A portions of the LPS. The sensitivity of exo5 mutant rhizobia to hydrophobic compounds shows the involvement of the galacturonic acid residues in the outer membrane structure. Nodulation studies with Vicia sativa subsp. nigra showed that exo5 mutant rhizobia are impaired in successful infection thr...Continue Reading

References

May 1, 1992·Journal of Bacteriology·A Urzainqui, G C Walker
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·L BattistiJ A Leigh
Jul 1, 1990·Applied and Environmental Microbiology·M W BreedveldA J Zehnder
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J A LeighG C Walker
Sep 1, 1974·Journal of General Microbiology·J E Beringer
May 14, 1969·Journal of Molecular Biology·H W Boyer, D Roulland-Dussoix
Jan 1, 1984·Annual Review of Microbiology·R E Hancock
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G DittaD R Helinski
May 19, 1995·The Journal of Biological Chemistry·R W CarlsonK D Noel
Jun 1, 1994·Microbiological Reviews·M W Breedveld, K J Miller
Feb 1, 1993·Journal of Bacteriology·M W BreedveldA J Zehnder
Dec 11, 1999·Biochimica Et Biophysica Acta·R M Epand, H J Vogel
Dec 21, 2000·Current Opinion in Microbiology·D J Gage, W Margolin
Nov 10, 2001·Annual Review of Phytopathology·Max DowEdda von Roepenack
Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Gordon R O CampbellGraham C Walker
May 25, 2002·Molecular Plant-microbe Interactions : MPMI·Anton A N van BrusselJan W Kijne
May 25, 2002·Molecular Plant-microbe Interactions : MPMI·Elizabeth A RathbunNicholas J Brewin
Jun 5, 2002·Annual Review of Biochemistry·Christian R H Raetz, Chris Whitfield
Jul 24, 2002·Clinica Chimica Acta; International Journal of Clinical Chemistry·Didier Heumann, Thierry Roger
Jan 18, 2003·The Journal of Biological Chemistry·Nanette L S Que-GewirthChristian R H Raetz

❮ Previous
Next ❯

Citations

Jul 19, 2012·World Journal of Microbiology & Biotechnology·K H HuangC C Young
Feb 25, 2006·The Journal of Biological Chemistry·Suparna Kanjilal-KolarChristian R H Raetz
Nov 9, 2012·Molecular Plant-microbe Interactions : MPMI·Simon J KellyClive W Ronson
Dec 16, 2005·Molecular Plant-microbe Interactions : MPMI·Marc C LausJan W Kijne
Jul 5, 2008·Plant Physiology·Griet Den HerderSofie Goormachtig
Feb 18, 2006·Microbial Cell Factories·Anna SkorupskaJaroslaw Król
Jan 1, 2010·Microbes and Environments·Ken-Ichi KuchoPhilippe Normand
Apr 20, 2011·Microbes and Environments·Naoganchaolu BorjiginHisayuki Mitsui
Dec 17, 2011·International Journal of Molecular Sciences·Monika Janczarek
Dec 5, 2015·Plant Signaling & Behavior·Virginia Dalla ViaFlavio Blanco
Mar 24, 2006·Molecular Microbiology·Marc C LausJan W Kijne
Nov 10, 2012·Biochemical and Biophysical Research Communications·Ana BarbasArsénio M Fialho
Jan 17, 2015·International Journal of Biological Macromolecules·Tereza Cristina Luque CastellaneEliana Gertrudes de Macedo Lemos
Nov 26, 2009·FEMS Microbiology Letters·Luciana V Rinaudi, Walter Giordano
May 27, 2016·Archives of Microbiology·Agustín Reyes-PérezSergio Encarnacion
Apr 14, 2017·Journal of Basic Microbiology·Kulandaivelu VelmourouganeAnil Kumar Saxena
Sep 28, 2018·Frontiers in Microbiology·María J LoriteMilagros León-Barrios
Mar 10, 2016·Journal of Plant Research·Ken-Ichi OsukiToshiki Uchiumi
Jul 3, 2021·International Journal of Molecular Sciences·Sebastián Acosta-JuradoJosé-María Vinardell

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.