Involvement of FAK/Src complex in the processes of Escherichia coli phagocytosis by insect hemocytes

FEBS Letters
Aristea MethenitiV J Marmaras

Abstract

Recently we demonstrated that lipopolysaccharide promotes activation of the Ras/mitogen-activated protein cascade in hemocytes and that phagocytosis of Escherichia coli by insect hemocytes is mediated by an integrin-dependent process [Foukas et al. (1998) J. Biol. Chem. 273, 14813--14818]. Here we report data concerning the focal adhesion kinase (FAK) tyrosine phosphorylation status in hemocytes in response to E. coli. We demonstrate that E. coli-triggering stimulates a significant increase in tyrosine phosphorylation of FAK in hemocytes. Furthermore, immunoblotting analysis using anti-Y397 demonstrated intense FAK activity at the Y397/SH2-binding site in hemocytes treated with E. coli. In addition, antibody-mediated inhibition of FAK and Src-kinase has been shown to abolish FAK phosphorylation and E. coli phagocytosis, indicating a specific role for the FAK/Src complex in the processes of promoting cell phagocytosis. These findings expand the known signaling functions of FAK and provide insight into signal transduction events associated with hemocyte phagocytosis in response to E. coli.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 15, 1995·The Biochemical Journal·S GhazizadehH B Fleit
Feb 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·E J Brown
Aug 26, 1994·Cell·J V Ravetch
Jan 1, 1995·Annual Review of Cell and Developmental Biology·M A SchwartzM H Ginsberg
Sep 1, 1996·Insect Biochemistry and Molecular Biology·N D CharalambidisV J Marmaras
Jan 1, 1997·Annual Review of Entomology·J P GillespieT Trenczek
Feb 1, 1997·Current Opinion in Immunology·J V Ravetch
May 23, 1998·Annual Review of Immunology·J V Ravetch, R A Clynes
Jun 4, 1999·Progress in Biophysics and Molecular Biology·D D SchlaepferD J Sieg
Jun 8, 1999·Annual Review of Immunology·A Aderem, D M Underhill
Dec 2, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J L Rodríguez-Fernández
Dec 10, 1999·The Journal of Biological Chemistry·R H PalmerT Hunter
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·G L FoxR O Hynes
Mar 1, 1995·Trends in Cell Biology·S Greenberg

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Citations

Sep 13, 2002·Insect Biochemistry and Molecular Biology·M D Lavine, M R Strand
Oct 18, 2003·Insect Biochemistry and Molecular Biology·Anastasios N SoldatosVassilis J Marmaras
May 20, 2011·The Journal of Biological Chemistry·Kaz NagaosaYoshinobu Nakanishi
Aug 17, 2012·Proceedings. Biological Sciences·C V Palmer, N Traylor-Knowles
Mar 8, 2005·Immunology and Cell Biology·Cory L Brooks, Gary B Dunphy
Jun 1, 2005·Biochimica Et Biophysica Acta·Maria D MavrouliVassilis J Marmaras
Sep 16, 2008·Cellular Signalling·Vassilis J Marmaras, Maria Lampropoulou
Aug 28, 2003·Archives of Insect Biochemistry and Physiology·Aristea MethenitiMaria Lambropoulou
Oct 24, 2007·Journal of Cellular Biochemistry·Irene MamaliVassilis J Marmaras
Jun 30, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anongnard KasornHongbo R Luo
May 4, 2020·Experimental Cell Research·Jifei MiaoHui Li
Mar 14, 2002·International Journal of Medical Microbiology : IJMM·Maria FällmanKaren McGee
May 16, 2006·Immunobiology·Pikul JiravanichpaisalKenneth Söderhäll

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