Involvement of Fas in the apoptosis of mouse germ cells induced by experimental cryptorchidism

Urological Research
S OgiN Terada

Abstract

The role of Fas in the apoptosis of testicular germ cells was investigated in BALB/c mice and Fas-deficient lpr/lpr mice. Spontaneous apoptosis of germ cells was observed in the testes of 40-day-old BALB/c mice, and experimentally induced cryptorchidism increased this apoptosis to such an extent that there was a decrease in the weight of the testis. Flow cytometry and immunohistochemistry using a Fas-specific monoclonal antibody demonstrated expression of Fas on germ cells including spermatogonia, spermatocytes, and spermatids. Furthermore, analysis by flow cytometry suggested that Fas expression on germ cells was increased following cryptorchidism. However, spontaneous and cryptorchidism-induced apoptosis of germ cells were also observed in 40-day-old Fas-deficient lpr/lpr mice. Moreover, testis weight also decreased following cryptorchidism in the mutant mice. The present results may indicate that the expression of Fas on germ cells does not correlate with spontaneous apoptosis or apoptosis induced by cryptorchidism. However, on the contrary, this study shows that Fas are partly involved in cryptorchidism-induced apoptosis, because the decrease in testis weight of lpr/lpr mice was less than that in BALB/c mice.

Citations

Oct 1, 2005·Pathology Oncology Research : POR·Mehmet DündarHaluk Erol
Feb 6, 2008·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Claudia HernandezJune K Robinson
Jan 6, 2000·American Journal of Reproductive Immunology : AJRI·J P XuT Mori
Mar 26, 2003·Cell Structure and Function·Kazuhiro Sakamaki
Apr 22, 2003·Archives of Histology and Cytology·Takehiko Koji, Yoshitaka Hishikawa
Aug 1, 2009·Journal of Biomedicine & Biotechnology·Yuqin ShiKedi Yang
Dec 6, 2011·Reproductive Biology·Aniela GolasJozefa Styrna
Jan 4, 2007·International Journal of Urology : Official Journal of the Japanese Urological Association·Kentaro MizunoKenjiro Kohri
Apr 12, 2011·Molecular Reproduction and Development·Fang LiuHui Huang
Jun 7, 2002·The Journal of Urology·Hiroshi TomomasaHiromichi Ishikawa
Dec 5, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·R-Marc PelletierMaría Leiza Vitale
Jan 6, 2006·Acta Biochimica Et Biophysica Sinica·Yun DengGuang-Xiu Lu
Jan 10, 2002·Journal of Andrology·Yizhong YinAbraham Morgentaler
Mar 20, 2019·Zygote : the Biology of Gametes and Early Embryos·Yi ZhengWenxian Zeng

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis