Involvement of fibrinogen in protamine-induced pulmonary hypertension

The Journal of Surgical Research
M Sogawa, S F Mohammad

Abstract

Protamine reversal of heparin anticoagulation occasionally results in pulmonary hypertension as well as systemic hypotension. To examine the contribution of blood components to this induction of pulmonary hypertension, we developed an isolated rat lung perfusion model and perfused heparinized plasma, heparinized serum, and Hepes (4% bovine serum albumin, 20 mM N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid, 5 mM glucose, in warm physiological saline) buffer solution with or without fibrinogen. Perfusion with heparinized plasma and Hepes buffer solution with fibrinogen caused pulmonary hypertension; perfusion with heparinized serum or Hepes buffer solution without fibrinogen did not, suggesting that fibrinogen is involved in the induction of pulmonary hypertension. We also labeled protamine with 125I and compared the amounts of protamine accumulating in the lung with different concentrations of fibrinogen. The amount of protamine trapped in the lung increased according to the concentration of fibrinogen. Fibrinogen may accelerate the reaction between pulmonary endothelial cells and protamine or protamine-heparin complexes. In the mechanism of protamine-induced pulmonary hypertension, fibrinogen, as well as heparin and prota...Continue Reading

References

Jun 1, 1991·Journal of Cardiothoracic and Vascular Anesthesia·R De PaulisD B Olsen
Apr 1, 1987·Thrombosis Research·C E Dempfle, D L Heene
Dec 1, 1983·The Journal of Thoracic and Cardiovascular Surgery·J K KirklinA D Pacifico
Mar 1, 1984·Clinical Cardiology·C L HollandW Fang
Jul 1, 1995·Anesthesia and Analgesia·T S HakimE M Camporesi

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Citations

Feb 5, 2015·Archivos de cardiología de México·Juan Calderón-ColmeneroMiguel Beltrán Gámez
Sep 8, 2017·Biomacromolecules·Manu Thomas KalathottukarenJayachandran N Kizhakkedathu

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